64691-29-8Relevant academic research and scientific papers
Effect of acyl chain length on selective biocatalytic deacylation on O-aryl glycosides and separation of anomers
Aggarwal, Neha,Arya, Anu,Mathur, Divya,Singh, Sukhdev,Tyagi, Abhilash,Kumar, Rajesh,Rana, Neha,Singh, Rajendra,Prasad, Ashok K.
, p. 83 - 91 (2014/04/03)
It has been demonstrated that Lipozyme TL IM (Thermomyces lanuginosus lipase immobilised on silica) can selectively deacylate the ester function involving the C-5′ hydroxyl group of α-anomers over the other acyl functions of anomeric mixture of peracylate
O-Aryl α,β-d-ribofuranosides: Synthesis & highly efficient biocatalytic separation of anomers and evaluation of their Src kinase inhibitory activity
Sharma, Raman K.,Singh, Sukhdev,Tiwari, Rakesh,Mandal, Deendayal,Olsen, Carl E.,Parmar, Virinder S.,Parang, Keykavous,Prasad, Ashok K.
, p. 6821 - 6830 (2013/01/15)
A series of peracetylated O-aryl α,β-d-ribofuranosides have been synthesized and an efficient biocatalytic methodology has been developed for the separation of their anomers which was otherwise almost impossible by column chromatographic or other techniques. The incubation of 2,3,5-tri-O-acetyl-1-O- aryl-α,β-d-ribofuranoside with Lipozyme TL IM immobilized on silica led to the selective deacetylation of only one acetoxy group, viz the C-5′-O-acetoxy group of the α-anomer over the other acetoxy groups derived from the two secondary hydroxyl groups present in the molecule and also over three acetoxy groups (derived from one primary and two secondary hydroxyls of the β-anomer). This methodology led to the easy synthesis of both, α- and β-anomers of O-aryl d-ribofuranosides. All the arylribofuranosides were screened for inhibition of Src kinase. 1-O-(3-Methoxyphenyl)-β-d-ribofuranoside exhibited the highest activity for inhibition of Src kinase (IC50 = 95.0 μM).
ENZYME DETECTION/ASSAY METHOD AND SUBSTRATES
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, (2008/06/13)
The invention relates to a method of detecting and/or assaying nucleoside hydrolases or nucleoside phosphorylases using a chromogenic substrate. Preferred chromogenic substrates have formula (I) where X is OH, or H, and Y is the residue of Y—OH where Y—OH is a chromophore or a compound readily converted to a chromophore and the substrates are hydrolysed by the nucleoside hydrolase to yield ribose or 2-deoxyribose plus Y—OH. Alternatively those substrates may be phosphorylysed by nucleoside phosphorylase to yield ribose-1-phosphate plus Y—OH. The methods may be used to detect and/or assay parasites in biological samples.
