65456-04-4Relevant articles and documents
An Fe2+- and α-Ketoglutarate-Dependent Halogenase Acts on Nucleotide Substrates
Deng, Zixin,Li, Qin,Yan, Shan,Ye, Ying,Zhang, Yonghui,Zhao, Chunhua,Zhong, Zhiyu,Zhu, Hucheng
, p. 9478 - 9484 (2020)
While halogenated nucleosides are used as common anticancer and antiviral drugs, naturally occurring halogenated nucleosides are rare. Adechlorin (ade) is a 2′-chloro nucleoside natural product first identified from Actinomadura sp. ATCC 39365. However, the installation of chlorine in the ade biosynthetic pathway remains elusive. Reported herein is a Fe2+-α-ketoglutarate halogenase AdeV that can install a chlorine atom at the C2′ position of 2′-deoxyadenosine monophosphate to afford 2′-chloro-2′-deoxyadenosine monophosphate. Furthermore, 2′,3′-dideoxyadenosine-5′-monophosphate and 2′-deoxyinosine-5′-monophosphate can also be converted, albeit 20-fold and 2-fold, respectively, less efficiently relative to the conversion of 2′-deoxyadenosine monophosphate. AdeV represents the first example of a Fe2+-α-ketoglutarate-dependent halogenase that converts nucleotides into chlorinated analogues.
