663625-87-4Relevant academic research and scientific papers
In vivo imaging of near-membrane calcium ions with two-photon probes
Lim, Chang Su,Kang, Min Young,Han, Ji Hee,Danish, Isravel Antony,Cho, Bong Rae
, p. 2028 - 2033 (2011)
We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
