669776-71-0

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 647852-34-4 5-(4-benzoylthio-1-butynyl)-2'-deoxycytidine 
• 98-91-9 thiobenzoic acid 
• 211510-45-1 but-1-yn-4-yl thiobenzoate 
• 845620-48-6 1-[2-deoxy-5-O-(4,4′-dimethoxytrityl)-β-D-erythro-pentofuranosyl]-5-iodocytosine 
• 611-53-0 5-Iodo-2'-deoxycytidine 

Downstream Products

• 669776-73-2 4-[3'-O,4-N-diacetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidin-5-yl]but-3-ynyl tert-butyl disulfide 
• 669776-75-4 4-[3'-O-acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidin-5-yl]but-3-ynyl tert-butyl disulfide 
• 647852-37-7 4-[5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidin-5-yl]but-3-ynyl tert-butyl disulfide 

Relevant academic research and scientific papers

2'-deoxycytidines carrying amino and thiol functionality: synthesis and incorporation by Vent (exo-) polymerase.

[reaction: see text] The synthesis of 2'-deoxycytidine nucleosides bearing amino and thiol groups appended to the 5-position of the nucleobase via a butynyl linker is described. The corresponding triphosphates were then synthesized from the nucleoside and incorporated into oligonucleotides by Vent (exo(-)) DNA polymerase. The ability of Vent (exo(-)) polymerase to amplify oligonucleotides containing these functionalized cytidine derivatives in a polymerase chain reaction (PCR) was demonstrated for the amino-functionalized derivative.

C-5 Modified nucleosides: Direct insertion of alkynyl-thio functionality in pyrimidines

A route is presented to append, in a single step, alkynyl thioesters to the 5-position of a pyrimidine ring of a nucleoside that is unprotected. These products should be useful to support in vitro selection experiments with functionalized DNA.