68631-48-1Relevant academic research and scientific papers
ENZYME ACTIVITIES IN CELL-FREE EXTRACTS OF SHIKONIN-PRODUCING LITHOSPERMUM ERYTHRORHIZON CELL SUSPENSION CULTURES
Heide, Lutz,Tabata, Mamoru
, p. 1645 - 1650 (1987)
Lithospermum erythrorhizon cell cultures excrete large quantities of naphthoquinone pigments, viz. shikonin derivatives, which have made enzymatic studies impossible so far.This paper describes methods for the removal of shikonin derivatives from the cells with liquid paraffin during culture growth and for the preparation of active enzyme extracts.Chorismate mutase and shikimate dehydrogenase activities were used as indicators for the preparation of active extracts.The efficacy of the methods developed was proved by the demonstration of the enzymatic formation of m-geranyl-p-hydroxybenzoic acid from geranylpyrophosphate and p-hydroxybenzoic acid in cell-free extracts of L. erythrorhizon cultures, the first detection of a key enzyme of shikonin biosynthesis.Key Word Index - Lithospermum erythrorhizon; Boraginaceae; cell culture; naphthoquinones; shikonin; chorismate mutase; shikimate dehydrogenase.
Identification of a bacteria-produced benzisoxazole with antibiotic activity against multi-drug resistant Acinetobacter baumannii
Deering, Robert W.,Whalen, Kristen E.,Alvarez, Ivan,Daffinee, Kathryn,Beganovic, Maya,LaPlante, Kerry L.,Kishore, Shreya,Zhao, Sijing,Cezairliyan, Brent,Yu, Shen,Rosario, Margaret,Mincer, Tracy J.,Rowley, David C.
, p. 370 - 380 (2021/02/22)
The emergence of multi-drug resistant pathogenic bacteria represents a serious and growing threat to national healthcare systems. Most pressing is an immediate need for the development of novel antibacterial agents to treat Gram-negative multi-drug resistant infections, including the opportunistic, hospital-derived pathogen, Acinetobacter baumannii. Herein we report a naturally occurring 1,2-benzisoxazole with minimum inhibitory concentrations as low as 6.25 μg ml?1 against clinical strains of multi-drug resistant A. baumannii and investigate its possible mechanisms of action. This molecule represents a new chemotype for antibacterial agents against A. baumannii and is easily accessed in two steps via de novo synthesis. In vitro testing of structural analogs suggest that the natural compound may already be optimized for activity against this pathogen. Our results demonstrate that supplementation of 4-hydroxybenzoate in minimal media was able to reverse 1,2-benzisoxazole’s antibacterial effects in A. baumannii. A search of metabolic pathways involving 4-hydroxybenzoate coupled with molecular modeling studies implicates two enzymes, chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyltransferase, as promising leads for the target of 3,6-dihydroxy-1,2-benzisoxazole.
PHENYL-PRENYL DERIVATIVES, OF MARINE AND SYNTHETIC ORIGIN, FOR THE TREATMENT OF COGNITIVE, NEURODEGENERATIVE OR NEURONAL DISEASES OR DISORDERS
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Page/Page column 37, (2009/10/09)
The present invention is related to a family of phenyl-prenyl derivatives of formula (I), and to their use in the treatment of cognitive, neurodegenerative or neuronal diseases or disorders, such as Alzheimer's disease or Parkinson's Disease. The present
