68985-14-8

Upstream product

• 572-09-8 2,3,4,6-tetra-O-acetyl-α-D-glucopyranosyl bromide 
• 121-34-6 3-methoxy-4-hydroxybenzoic acid 
• 2492-87-7 4-nitrophenyl-β-D-glucoside 
• 10543-12-1 4-acetoxy-3-methoxybenzoic acid 
• 58860-84-7 4-(benzoyloxy)benzoyl chloride 
• 133-89-1 UDP-glucose 

Downstream Products

• 20905-74-2 3,5-di-O-β-D-glucopyranoside of cyanidin 

Relevant academic research and scientific papers

Engineering faster transglycosidases and their acceptor specificity

Transglycosidases are enzymes that have the potential to catalyze the synthesis of a wide range of high-value compounds starting from biomass-derived feedstocks. Improving their activity and broadening the substrate range are important goals to enable the widespread application of this family of biocatalysts. In this work, we engineered 20 mutants of the rice transglycosidase Os9BGlu31 and evaluated their catalysis in 462 reactions over 18 different substrates. This allowed us to identify mutants that expanded their substrate range and showed high activity, including W243L and W243N. We also developed double mutants that show very high activity on certain substrates and exceptional specificity towards hydrolysis, such as L241D/W243N. In order to guide a more general use of Os9BGlu31 variants as transglycosylation catalysts, we built cheminformatics models based on topological descriptors of the substrates. These models showed useful predictive potential on the external validation set and are allowing the identification of efficient catalytic routes to novel phytohormone and antibiotic glucoconjugates of interest.

NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND

An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R 1 is independently selected from hydrogen, or C 1-6 alkyl, C 2-6 alkenyl, and C 2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO 2 , and SO 2 , n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a 2 bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

GLUCOSIDES AND GLUCOSE ESTERS OF HYDROXYBENZOIC ACIDS IN PLANTS

Pinaceae; Brassicaceae; Solanaceae; Lamiaceae; hydroxybenzoic acid glucosides; hydroxybenzoylglucoses; glucose derivatives.Six hydroxybenzoic acid glucosides and three hydroxybenzoylglucoses were synthesized as reference substances and their content in plant extracts was determined by means of capillary GC and HPLC.The results suggest a wide distribution for the glucosides, whereas the glucose esters occur less frequently.