6976-37-0

Basic information

• Product Name: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
• Synonyms: 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE;2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL;2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol;Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane;Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade;Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS;Bis(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methane biological buffer;bis-tris cell culture tested
• CAS NO: 6976-37-0
• Molecular Formula: C₈H₁₉NO₅
• Molecular Weight: 209.24
• EINECS: 230-237-7
• Product Categories: API intermediates;Buffer
• Mol File: 6976-37-0.mol
• Article Data: 0

Chemical Properties

• Melting Point: 104 °C(lit.)
• Boiling Point: 466.2 °C at 760 mmHg
• Flash Point: 288 °C
• Appearance: Yellow to orange/Solution
• Density: 1.346 g/cm³
• Vapor Pressure: 1.19E-10mmHg at 25°C
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear, colorless
• PKA: 6.5(at 25℃)
• Water Solubility: soluble
• Sensitive: Air Sensitive
• BRN: 2205275
• CAS DataBase Reference: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(CAS DataBase Reference)
• NIST Chemistry Reference: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(6976-37-0)
• EPA Substance Registry System: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(6976-37-0)

Safety Data

• Hazard Codes: Xi,Xn
• Statements: 36/37/38-21/22-20/21/22
• Safety Statements: 26-36-36/37/39-22
• WGK Germany: 3
• F: 10-23
• TSCA: Yes
• Hazardous Substances Data: 6976-37-0(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 6976-37-0 differently. You can refer to the following data:
1. It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin.
2. Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Flammability and Explosibility

Notclassified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

InChI:InChI=1/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2/p+1