7013-03-8Relevant academic research and scientific papers
Determination of Formate in Natural Waters by a Coupled Enzymatic/High-Performance Liquid Chromatographic Technique
Kieber, David J.,Vaughan, Graham M.,Mopper, Kenneth
, p. 1654 - 1659 (1988)
An enzymatic method was developed to quantify formic acid in natural water samples at submicromolar concentrations.The method is based on the oxidation of formate by formate dehydrogenase with corresponding reduction of β-nicotinamide adenine dinucleotide (β-NAD+) to reduced β-NAD+ (β-NADH); β-NADH is quantified by reversed-phase high-performance liquid chromatograhy with fluorometric detection.An important feature of this method is that the enzymatic reaction occurs directly in aqueous media, even seawater, and does not require sample pretreatment other than sample filtration.The reaction proceeds at room temperature at a slightly alkaline pH (7.5-8.5) and is specific for formate with a detection limit of 0.5 μM (S/N = 4) for a 200-μL injection.The precision of the method was 4.6percent relative standard deviation (n = 6) for a 0.6 μM standard addition of formate to Sargasso seawater.Average recoveries of 2 μM additions of formate to seawater, porewater, or rain were 103, 103, and 87percent, respectively.Intercalibration with a Dionex ion chromatographic system showed an excellent agreement of 98percent.Concentrations of formate present in natural samples ranged from 0.2 to 0.8 μM for Biscayne Bay seawater, 0.4 to 10.0 μM for Miami rain, and 0.9 to 8.4 μM for Biscayne Bay sediment porewater.
TENTATIVES DE REGENERATION DU COENZYME NADH PAR REDUCTION ELECTROCHIMIQUE ET HYDROGENATION CATALYTIQUE
Bergel, Alain,Durliat, Helene,Comtat, Maurice
, p. 593 - 600 (2007/10/02)
Among the various attempts for the electrochemical reduction of NAD(1+) on a platinum electrode only those realized with an electrode submitted to repetitive potentiodynamic perturbations lead to NADH with very low rates.Hydrogenation by molecular hydrogen catalysed by platinum allows the transformation of about 50percent of NAD(1+) in β-NADH enzymatically active.The reaction is faster with platinized platinum.In both cases β-NADH evolves with time to give three non identified products.
