70290-86-7Relevant articles and documents
Light-enabled synthesis of anhydrides and amides
Mccallum, Terry,Barriault, Louis
, p. 2874 - 2878 (2015/03/30)
Recently, we have demonstrated that the photogeneration of Vilsmeier-Haack reagents is possible using only dimethylformamide (DMF) and tetrabromomethane (CBr4) in the bromination of alcohols. Extending these findings to carboxylic acid substrates has produced a mild and facile approach to the in situ formation of symmetric anhydrides, which were conveniently converted to amide derivatives in a one-pot process. The efficient protocols discussed herein are marked by use of UVA LEDs (365 nm), which have reduced the reaction times and come with a low setup cost.
POST-PROLINE ENDOPEPTIDASE. PARTIAL PURIFICATION AND CHARACTERIZATION OF THE ENZYME FROM PIG KIDNEYS
Hauzer, Karel,Servitova, Linda,Barth, Tomislav,Jost, Karel
, p. 1139 - 1148 (2007/10/02)
Post-proline endopeptidase was isolated from pig kidneys and partially purified.The procedure consisted of fractionation with ammonium sulphate, ion exchange chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-200 and rechromatography on DEAE-Sephadex A-50.The preparation had 55 times higher specific activity than the crude extract and did not contain any contaminating enzymic activities.The enzyme cleaved a number of proline-containing peptides and was strictly specific in catalyzing the hydrolysis of the peptide bond on the carboxyl side of the proline residue.The optimum pH for the hydrolysis of the synthetic peptides benzyloxycarbonylglycyl-prolyl-leucyl-glycinamide and benzyloxycarbonyl-glycyl-proline β-naphthylamide was 7.8-8.0 and, in the case of benzyloxycarbonylglycyl-proline p-nitroanilide, 7.2 to 7.5.For the hydrolysis of the tetrapeptide benzyloxycarbonylglycyl-prolyl-leucyl-glycinamide, the Km value of 75 μmol l-1 was obtained.
