70323-44-3

Basic information

• Product Name: PERTUSSIS TOXIN
• Synonyms: histamine-sensitizingfactor;iap;lymphocytosis-promotingfactor;pertussigen;pertussistoxins;toxins,pertussis;PTX;PERTUSSIS TOXIN
• CAS NO: 70323-44-3
• Molecular Formula: NULL
• Molecular Weight: 0
• Product Categories: G-Protein
• Mol File: 70323-44-3.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: PERTUSSIS TOXIN(CAS DataBase Reference)
• NIST Chemistry Reference: PERTUSSIS TOXIN(70323-44-3)
• EPA Substance Registry System: PERTUSSIS TOXIN(70323-44-3)

Safety Data

• Hazard Codes: T,B,Xi
• Statements: 23/24/25-36/37/38
• Safety Statements: 36/37/39-45-26
• RIDADR: UN 3462 6.1/PG 1
• WGK Germany: 3
• RTECS: XW5883750
• F: 10
• HazardClass: 6.1(a)
• PackingGroup: I
• Hazardous Substances Data: 70323-44-3(Hazardous Substances Data)

Usage

Description

Pertussis toxin, also known as whooping cough toxin, is a bacterial protein exotoxin produced by the Bordetella pertussis bacterium. It is a potent virulence factor that plays a significant role in the pathogenesis of whooping cough. The toxin is composed of multiple subunits, including the B oligomer and the A protomer, which become activated upon binding to the cell membrane.

Uses

Used in Research and Development:
Pertussis toxin is used as a research tool for studying the mechanisms of bacterial infection, toxin action, and the immune response. It helps researchers understand the interactions between the toxin and host cells, as well as the role of specific cellular pathways in the infection process.
Used in Vaccine Development:
Pertussis toxin is used in the development of vaccines against whooping cough. By understanding the structure and function of the toxin, scientists can design vaccines that target the toxin and provide immunity against the disease.
Used in Diagnostics:
Pertussis toxin can be used as a diagnostic marker for the detection of B. pertussis infections. The presence of the toxin in clinical samples can help confirm the diagnosis of whooping cough.
Used in Anticancer Applications:
Although not explicitly mentioned in the provided materials, pertussis toxin has been studied for its potential use in cancer therapy. It has been shown to modulate immune responses and has been investigated for its ability to enhance the effectiveness of certain cancer treatments.
Used in Drug Delivery Systems:
Pertussis toxin can be used as a component in drug delivery systems, particularly for targeted drug delivery. The toxin's ability to bind specifically to cell membranes can be exploited to deliver therapeutic agents directly to infected or cancerous cells, potentially improving the efficacy and reducing the side effects of the treatment.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.

Purification Methods

Purify the toxin by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and the purity is checked by SDS-PAGE. [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 1982, Biochem J 83 295 1978.]