71119-22-7

Basic information

• Product Name: MOPS sodium salt
• Synonyms: LABOTEST-BB LT01596055;3-(N-Morpholino)propanesulfonic acid sodium salt, 4-Morpholinepropanesulfonic acid sodium salt;3-Morpholino-1-propanesulfonic acid sodium salt;3-Morpholinopropane-1-sulfonic acid sodium salt;MOPS sodium salt ,99.5%;3-(Morpholin-4-yl)propanesulphonic acid, sodium salt >98%;MOPS Sodium Salt 3-Morpholinopropanesulfonic Acid Sodium Salt;3-(N-Morpholine)propane Sulphonic Acid,SodiuM
• CAS NO: 71119-22-7
• Molecular Formula: C7H14NNaO4S
• Molecular Weight: 231.25
• EINECS: 1533716-785-6
• Product Categories: Buffer
• Mol File: 71119-22-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: 277-282°C
• Appearance: White/Powder/Solid
• Density: 1.41[at 20℃]
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear, colorless
• PKA: 7.2(at 25℃)
• Water Solubility: Soluble in water (523 g/L at 20°C).
• CAS DataBase Reference: MOPS sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: MOPS sodium salt(71119-22-7)
• EPA Substance Registry System: MOPS sodium salt(71119-22-7)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 24/25-36-26
• WGK Germany: 1
• F: 10
• TSCA: Yes
• Hazardous Substances Data: 71119-22-7(Hazardous Substances Data)

Usage

Description

MOPS sodium salt, also known as 3-(N-morpholino) propanesulfonic acid sodium salt, is a zwitterionic, morpholinic buffering agent used in biochemistry and molecular biology. It is characterized by its white powder form and is effective for a pH range of 6.5 – 7.9. MOPS sodium salt is known for its ability to maintain a stable pH environment in various applications without forming complexes with most metal ions, making it a preferred choice as a non-coordinating buffer in solutions containing metal ions.

Uses

Used in Biochemistry and Molecular Biology:
MOPS sodium salt is used as a buffering agent for maintaining a stable pH environment in cell culture media, electrophoresis running buffers, and protein purification chromatography processes. Its zwitterionic nature and lack of metal ion complexation make it an ideal choice for these applications.
Used in Cell Culture Media:
MOPS sodium salt is used as a buffering agent in buffered culture media for various cell types, including bacteria, yeast, and mammalian cells. It helps maintain a consistent pH, which is crucial for optimal cell growth and function.
Used in Electrophoresis:
MOPS sodium salt is utilized as a running buffer in electrophoresis techniques, such as agarose gel electrophoresis, to separate RNA and other biomolecules based on their size and charge. Its buffering capacity ensures a stable pH throughout the process.
Used in Protein Purification:
In chromatography, MOPS sodium salt serves as a buffering agent during protein purification. Its non-coordinating properties with metal ions help prevent unwanted interactions and maintain protein integrity.
Used in Bicinchoninic Acid (BCA) Assay:
MOPS sodium salt is suitable for use in the BCA assay, a colorimetric method for determining protein concentration. Its buffering capacity helps maintain a stable pH environment, ensuring accurate and reliable results.
Used in pH Adjustment:
MOPS sodium salt can be mixed with MOPS free acid to achieve the desired pH in various solutions. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain the required pH level.
Sterilization:
It is recommended to sterilize MOPS buffers by filtration rather than autoclaving due to the formation of unknown yellow degradation products that may occur after autoclaving.

Flammability and Explosibility

Notclassified

Biological Activity

Multi purpose buffering agent used in biological research. Working pH range in aqueous solution: 6.5 - 7.9. Commonly used in cell culture media, as a running buffer for gel eletrophoresis, and within protein purification in chromatography.

InChI:InChI=1/C7H15NO4S.Na/c9-13(10,11)7-1-2-8-3-5-12-6-4-8;/h1-7H2,(H,9,10,11);/q;+1/p-1