7114-03-6 Usage
Uses
Used in Molecular Biology:
Methyl Green zinc chloride salt is used as a tracking dye in acid buffers for electrophoresis, aiding in the differentiation of bacteria and staining of nucleoli and mitochondria in tissues.
Used in Histology:
In the Methyl Green-Pyronin method, Methyl Green zinc chloride salt is used to demonstrate DNA and RNA in tissue sections, providing valuable insights into cellular structures and functions.
Used in Cell Viability Assessment:
Methyl Green zinc chloride salt serves as an exclusion viability stain for cells, helping researchers determine the health and viability of cell populations.
Used in Fluorimetric Assays and Flow Cytometry:
Methyl Green zinc chloride salt is also utilized in fluorimetric assays and flow cytometry, contributing to various research and diagnostic applications in the fields of biology and medicine.
References
http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh®ion=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html
Purification Methods
Crystallise the dye from hot water. [Beilstein 13 IV 2286.]
Check Digit Verification of cas no
The CAS Registry Mumber 7114-03-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,1,1 and 4 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 7114-03:
(6*7)+(5*1)+(4*1)+(3*4)+(2*0)+(1*3)=66
66 % 10 = 6
So 7114-03-6 is a valid CAS Registry Number.
InChI:InChI=1/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
