72052-96-1 Usage
Uses
Used in Antileukemic Applications:
5-aza-2'-deoxycytidine-5'-triphosphate is used as an antileukemic agent for the treatment of certain types of leukemia. It works by inhibiting DNA methylation, a process that can lead to the silencing of tumor suppressor genes and contribute to the development of cancer. By blocking this process, 5-Aza-dCTP can help reactivate these genes and slow down or halt the progression of the disease.
Used in Molecular Biology Research:
In the field of molecular biology, 5-aza-2'-deoxycytidine-5'-triphosphate is used as a research tool for studying the role of DNA methylation in gene regulation and its impact on various biological processes. It is often employed in combination with purified DNA polymerase alpha and DNA methylase from mammalian cells to investigate the mechanisms of DNA methylation and its effects on gene expression.
Used in Drug Development:
5-aza-2'-deoxycytidine-5'-triphosphate also has potential applications in the development of new drugs targeting DNA methylation-related diseases, such as cancer and other genetic disorders. By understanding the molecular mechanisms underlying these conditions, researchers can design more effective therapies that exploit the unique properties of 5-Aza-dCTP to modulate gene expression and counteract the effects of aberrant methylation patterns.
Used in Epigenetic Therapy:
5-aza-2'-deoxycytidine-5'-triphosphate is used as an epigenetic therapy agent, targeting the epigenetic modifications that contribute to the development and progression of various diseases, including cancer. By inhibiting DNA methylation, it can help reverse the epigenetic changes that lead to the silencing of tumor suppressor genes and the activation of oncogenes, potentially offering a novel approach to treating these conditions.
Used in Diagnostic Applications:
In addition to its therapeutic applications, 5-aza-2'-deoxycytidine-5'-triphosphate can also be employed in diagnostic assays to detect and monitor the methylation status of specific genes. This information can be valuable for predicting disease risk, assessing treatment response, and guiding personalized treatment strategies in various clinical settings.
Check Digit Verification of cas no
The CAS Registry Mumber 72052-96-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,2,0,5 and 2 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 72052-96:
(7*7)+(6*2)+(5*0)+(4*5)+(3*2)+(2*9)+(1*6)=111
111 % 10 = 1
So 72052-96-1 is a valid CAS Registry Number.
InChI:InChI=1/C8H15N4O13P3/c9-7-10-3-12(8(14)11-7)6-1-4(13)5(23-6)2-22-27(18,19)25-28(20,21)24-26(15,16)17/h3-6,13H,1-2H2,(H,18,19)(H,20,21)(H2,9,11,14)(H2,15,16,17)/t4-,5+,6+/m0/s1
72052-96-1Relevant academic research and scientific papers
Jansen, Robert S.,Rosing, Hilde,Schellens, Jan Hm,Beijnen, Jos H.
, p. 14 - 26 (2010)
We present a facile method to phosphorylate small amounts of nucleosides (0.05 mol) into mixtures of their 5′-mono-, di-, and triphosphates in a one-pot reaction. The nucleosides were first converted into their dichlorophosphates using a large excess (15-18 equivalents) of phosphorous oxychloride in trimethylphosphate. The large excess resulted in good dichlorophosphate yields (46-76%) for the four nucleosides tested. Upon the addition of tributylammonium-phosphate with additional tributylamine (20 equivalents both), the dichlorophosphate was converted into a mixture containing equal amounts of the mono-, di-, and triphosphate. The presented method was successfully applied to synthesize mixtures of stable isotope labeled nucleotides, which can be used as internal standards in quantitative mass spectrometric assays.
