Benzoic acid, 4-[(2,4,6-trinitrophenyl)amino]-, also known as Trinitrobenzenesulfonic acid or TNBS, is a chemical compound with the molecular formula C13H8N4O7S. It is a yellow crystalline solid that is soluble in water and organic solvents. TNBS is commonly used as a reagent in various analytical techniques, particularly for the quantification of primary amines and amino groups. It reacts with amines to form a yellow-colored product, which can be measured spectrophotometrically. This property makes it a valuable tool in the determination of protein concentration, enzyme activity, and other applications in biochemistry and molecular biology. Additionally, TNBS has been used in the synthesis of dyes and as a chemical warfare agent. Due to its potential hazards, it is important to handle TNBS with care and in accordance with safety regulations.
The CAS Registry Mumber 7221-18-3 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,2,2 and 1 respectively; the second part has 2 digits, 1 and 8 respectively. Calculate Digit Verification of CAS Registry Number 7221-18: (6*7)+(5*2)+(4*2)+(3*1)+(2*1)+(1*8)=73 73 % 10 = 3 So 7221-18-3 is a valid CAS Registry Number.