73120-97-5Relevant academic research and scientific papers
Making cyclic RNAs easily available
Frieden, Miriam,Grandas, Anna,Pedroso, Enrique
, p. 1593 - 1594 (1999)
A simple solid-phase procedure allows cyclic oligoribonucleotides to be obtained as long as the linear precursor attached to the support has a 2'-deoxyribonucleoside or a 2'-O-methylribonucleoside at the 3'-end.
Synthesis of cyclic di-nucleotidic acids as potential inhibitors targeting diguanylate cyclase
Ching, Shi Min,Tan, Wan Jun,Chua, Kim Lee,Lam, Yulin
experimental part, p. 6657 - 6665 (2010/10/21)
Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating the compound and product peaks with good resolution was optimized and applied to the analysis of the compounds. Results obtained show that cyclic di-inosinylic acid 1b demonstrates a stronger inhibition on Slr1143 than c-di-GMP and is a potential inhibitor for biofilm formation.
Cyclic oligoribonucleotides (RNA) by solid-phase synthesis
Micura, Ronald
, p. 2077 - 2082 (2007/10/03)
A novel solid-phase synthesis of small-to medium-sized cyclic RNA oligonucleotides is presented. A major advantage of the approach is the lack of restrictions on the sequence variety with respect to the four standard bases adenine, cytosine, guanine, and uracil. This has been demonstrated for cycles containing 2 to 21 nucleotide units. The approach allows fully automated assembly, and is related to a procedure known for the preparation of cyclic oligonucleotides in the DNA series (E. Alazzouzi, N. Escaja, A. Grandas, E. Pedroso, Angew. Chem. 1997, 109, 1564-1567; Angew. Chem. Int. Ed. Engl. 1997, 36, 1506-1508). It combines standard phosphoramidite chemistry for chain elongation and standard phosphotriester chemistry for ring closure. A key aspect of the method is use of the novel 2′-O-triisopropylsilyloxymethyl (TOM) protected RNA phosphoramidites (X. Wu, S. Pitsch, Nucleic Acids Res. 1998, 26, 4315-4323) instead of the classic tert-butyldimethyl silyl (TBDMS) protected amidites. Furthermore, the design of the final cleavage step is selective only for correctly cyclized oligoribonucleotides. This results, after deprotection, in HPLC profiles in which the crude oligonucleotide is represented by the major peak with typically more than 80% of the integrated area. The ring closure itself proceeds with an average yield of 15%.
