73231-43-3Relevant academic research and scientific papers
Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure
Hennig, Mirko,Scott, Lincoln G.,Sperling, Edit,Bermel, Wolfgang,Williamson, James R.
, p. 14911 - 14921 (2007)
Enzymatic synthesis methods for the fluorinated 5′-triphosphate analogues 5F-UTP and 5F-CTP have been developed to facilitate 19F-labeling of RNAs for biophysical studies. HIV-2 TAR RNAs were synthesized using these analogues by in vitro transcription reactions using T7 RNA polymerase. The uniform incorporation of 5F-U or 5F-C analogues into HIV-2 TAR RNA transcripts does not significantly alter the RNA structure or thermodynamic stability. Fluorine observed homonuclear 19F- 19F and heteronuclear 19F-1H NOE experiments providing selective distance information are presented and discussed. The availability of efficient synthesis of 5F-UTP, and for the first time, 5F-CTP, will facilitate the use of 5F-labeled RNAs in structural, ligand binding, and dynamic studies of RNAs using the advantages of 19F-labeling.
