73463-39-5

Basic information

• Product Name: 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
• Synonyms: CAPSO),3-(CyclohexylaMino)-2-hydroxy-1-propane;CAPSO (3-[CyclohexylaMino]-2-hydroxy-1-propanesulfonic acid(Free Acid);CAPSO Vetec(TM) reagent grade, >=99%;CAPSO;3-[CYCLOHEXYLAMINO]-2-HYDROXY-1-PROPANESULFONIC ACID;3-(CYCLOHEXYLAMINO)-2-HYDROXY-1-PROPANESULPHONIC ACID;3-(CYCLOHEXYLAMINO)-2-HYDROXYL-PROPANESULFONIC ACID;3-CYCLOHEXYLAMINO-2-HYDROXY-PROPANE-1-SULFONIC ACID
• CAS NO: 73463-39-5
• Molecular Formula: C₉H₁₉NO₄S
• Molecular Weight: 237.32
• EINECS: 2017-001-1
• Product Categories: Pharmaceutical Intermediates;Buffer
• Mol File: 73463-39-5.mol
• Article Data: 0

Chemical Properties

• Melting Point: 270-274°C
• Appearance: White/Powder
• Density: 1.29 g/cm³
• Refractive Index: 1.538
• Storage Temp.: Sealed in dry,Room Temperature
• Solubility: H2O: 0.1 g/mL, clear, colorless
• PKA: 9.6(at 25℃)
• Water Solubility: Soluble in water (10 g+ 90 ml).
• BRN: 5939234
• CAS DataBase Reference: 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid(CAS DataBase Reference)
• NIST Chemistry Reference: 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid(73463-39-5)
• EPA Substance Registry System: 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid(73463-39-5)

Safety Data

• Hazard Codes: Xi,Xn
• Statements: 20/21/22-36/37/38
• Safety Statements: 22-24/25-36-26
• WGK Germany: 3
• F: 3
• HazardClass: IRRITANT
• Hazardous Substances Data: 73463-39-5(Hazardous Substances Data)

Usage

Description

CAPSO is a useful secondary standard buffer. Second dissociation constant pK2 value of CAPSO has been measured. Useful pH range for CAPSO is 8.9 - 10.3.CAPSO is a biological and zwitterionic buffer commonly categorized as a Good's buffers. CAPSO has many applications some of which include immunoblotting and westerns. Additionally, CAPSO is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. CAPS buffer has very low metal-binding constants and are, therefore, particularly suited to investigate metal-dependent enzymes. CAPSO a very water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins with minimal salt effects.

Chemical Properties

white powder

Uses

Different sources of media describe the Uses of 73463-39-5 differently. You can refer to the following data:
1. CAPSO was used as biological buffer in the following studies:To maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures.Chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes. Preparation of planar bilayer lipid membranes.It may be employed as buffer for the electrophoretic analyses in non-denaturing gels, to investigate macromolecular self-association of ADP-ribosyltransferase protein in solution.
2. CAPSO is used in various biological applications such as electrophoresis. Specific applications include buffers for protein separations and diagnostic reagent production. Also used as biological buffer in the following studies, to maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures, chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes, preparation of planar bilayer lipid membranes.

General Description

CAPSO is a useful secondary standard buffer. Second dissociation constant pK2 value of CAPSO has been measured. Useful pH range for CAPSO is 8.9 - 10.3.

InChI:InChI=1/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)/t9-/m1/s1