742079-15-8Relevant academic research and scientific papers
(R)-2,4-Dihydroxybutyramide seco-Pseudonucleosides: New Versatile Homochiral Synthons for Synthesis of Modified Oligonucleotides
Dioubankova, Natalia N.,Malakhov, Andrey D.,Stetsenko, Dmitry A.,Korshun, Vladimir A.,Gait, Michael J.
, p. 4607 - 4610 (2002)
(Matrix Presented) Two series of seco-pseudonucleoside synthons were synthesized from (R)-(+)-α-hydroxy-γ-butyrolactone and (R)-(-)-pantolactone by aminolysis, side-chain protection, dimethoxytritylation, and phosphitylation or solid-phase attachment. The phosphoramidites and solid supports were used in automated DNA synthesis to prepare oligonucleotides modified with one or more 2,4-dihydroxybutyramide units bearing side-chain reporter groups. These new oligonucleotide modification reagents allow the introduction of a label into any desired position within an oligonucleotide chain during solid-phase assembly.
Phosphoramidites and solid supports based on N-substituted 2,4-dihydroxybutyramides: universal reagents for synthesis of modified oligonucleotides
Dioubankova, Natalia N.,Malakhov, Andrei D.,Stetsenko, Dmitry A.,Gait, Michael J.,Korshun, Vladimir A.
, p. 6762 - 6773 (2007/10/03)
A general and convenient method for synthesis of modified oligonucleotides by use of new non-nucleoside phosphoramidites is reported. A chiral 1,3-diol backbone of the modifying reagents is generated either from (R)-(+)-α-hydroxy-γ-butyrolactone or (R)-(-)-pantolactone. Aliphatic amines were acylated with the lactones to give the corresponding N-substituted 2,4-dihydroxybutyramides. After protection of a side chain, if necessary, the diols were converted into phosphoramidites or solid supports suitable for use in oligonucleotide synthesis. The reagents allow single, multiple or combined introduction of various functions (e.g., alkylamine, imidazole and pyrene residues) into synthetic oligonucleotides. The structures of the conjugates were confirmed by MALDI-TOF mass spectrometry.
