76068-39-8Relevant academic research and scientific papers
Solvent-dependent ratiometric fluorescent merocyanine dyes: Spectral properties, interaction with BSA as well as biological applications
Gao, Dengfeng,Li, Anyang,Guan, Li,Zhang, Xianghan,Wang
, p. 163 - 173 (2016/03/15)
A series of merocyanine dyes with excellent spectral properties were synthesized through changing the heterocyclic base structure for protein labeling and imaging in biological science, such as living cells and living mice. Dyes 7c-7h showed solvent-dependent changes on emission wavelength and fluorescence intensity in water and hydrophobic solvents, which were further analyzed by theoretical calculations and absorption spectra. The interaction of dye 7d with bovine serum albumin (BSA) showed an obvious ratiometric fluorescence response to solvent/protein environment, with the potential to report protein conformational changes. Investigation of the cytotoxicity and bioimaging capability displayed that dyes 7b-7h showed low cytotoxicity, and could stain living cell cytoplasm and be used for imaging in living mice with bright fluorescence at the application dose, which was suggested as fluorescent reagents for imaging in biological science.
