76224-00-5Relevant academic research and scientific papers
Application of inverse substrates to trypsin-catalyzed peptide synthesis
Itoh, Kunihiko,Sekizaki, Haruo,Toyota, Eiko,Fujiwara, Norihisa,Tanizawa, Kazutaka
, p. 59 - 68 (1996)
Trypsin-catalyzed peptide synthesis has been studied by using 'inverse substrate,' i.e., p-amidinophenyl ester derived from α-amino acid derivative as an acyl donor component. Inverse substrate can afford acyl trypsin in a very specific manner, liberating
INVERSE SUBSTRATES IX. AMIDINOPHENYL ESTERS DERIVED FROM AMINO ACIDS AND PEPTIDES : SYNTHESIS AND PROPERTIES AS TRYPSIN SUBSTRATES
Fujioka, Toshiyuki,Tanizawa, Kazutaka,Nakayama, Hitoshi,Kanaoka, Yuichi
, p. 1899 - 1905 (2007/10/02)
Methods for preparation of p-amidinophenyl esters from amino acid derivatives are described.Blocking of the amidino function with the benzyloxycarbonyl group and its deblocking by catalytic hydrogenation after coupling were satisfactory. p-Amidino-phenyl esters are of special interest because of their susceptibility to the hydrolytic enzyme, trypsin, and some parameters of the hydrolysis of these compounds by tripsin are presented.Keywords - p-amidinophenyl ester; synthetic substrates; ester formation; trypsin; enzyme kinetics; deacylation rate constant
