76877-33-3

Basic information

• Product Name: 7-DIETHYLAMINO-3-(4-MALEIMIDOPHENYL)-4-METHYLCOUMARIN
• Synonyms: CPM;AURORA KA-6719;7-DIETHYLAMINO-3-(4-MALEIMIDOPHENYL)-4-METHYLCOUMARIN;7-DIETHYLAMINO-3-(4'-MALEIMIDYLPHENYL)-4-METHYLCOUMARIN;7-DIETHYLAMINO-3-(4-MALEIMIODOPHENYL)-4-METHYLCOUMARIN;1H-PYRROLE-2,5-DIONE, 1-(4-(7-(DIETHYLAMINO)-4-METHYL-2-OXO-2H-1-BENZOPYRAN-3-YL)PHENYL)-;n-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide;1-[4-(7-Diethylamino-4-methyl-2-oxo-2H-chromen-3-yl)-phenyl]-pyrrole-2,5-dione
• CAS NO: 76877-33-3
• Molecular Formula: C24H22N2O4
• Molecular Weight: 402.44
• Product Categories: Coumarines
• Mol File: 76877-33-3.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 618.7 °C at 760 mmHg
• Flash Point: 328 °C
• Appearance: yellow/
• Density: 1.302 g/cm³
• Vapor Pressure: 3.09E-15mmHg at 25°C
• Refractive Index: 1.648
• Storage Temp.: ?20°C
• Solubility: DMSO: soluble
• PKA: 3.28±0.20(Predicted)
• CAS DataBase Reference: 7-DIETHYLAMINO-3-(4-MALEIMIDOPHENYL)-4-METHYLCOUMARIN(CAS DataBase Reference)
• NIST Chemistry Reference: 7-DIETHYLAMINO-3-(4-MALEIMIDOPHENYL)-4-METHYLCOUMARIN(76877-33-3)
• EPA Substance Registry System: 7-DIETHYLAMINO-3-(4-MALEIMIDOPHENYL)-4-METHYLCOUMARIN(76877-33-3)

Safety Data

• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 76877-33-3(Hazardous Substances Data)

Usage

Description

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin is a thiol-reactive fluorescent probe. It displays excitation/emission maxima of 387/468 nm, respectively, and fluorescence intensity increases when bound to cysteine residues.

Uses

Different sources of media describe the Uses of 76877-33-3 differently. You can refer to the following data:
1. (7-Diethylamino-3-(4'-maleimidylphenyl)-4- methylcoumarin)CPM is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. CPM is a good energ
2. 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin is a thiol-reactive fluorescent probe. 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) is a thiol-reactive fluorescent probe. CPM selectively forms Michael adducts in the presence of hyperreactive sulfhydryls. CPM has been used to monitor the release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining. Dyes and metabolites.

InChI:InChI=1/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3