76973-77-8Relevant academic research and scientific papers
Unexpected reactivity of the 2′-carboxyl functionality in rhodamine dyes
Haack, Richard A.,Gayda, Susan,Himmelsbach, Richard J.,Tetin, Sergey Y.
, p. 1733 - 1737 (2017)
Contrary to previous literature reports, the reactivity of the 2′ carboxylic acid in rhodamine dyes was found to be much more reactive than anticipated. Typically, the 4′- or 5′-carboxy functionality in dicaboxyrhodamines is targeted for bioconjugation use due to the supposed unreactivity of the 2′ carboxylic acid. Reactive esters of 2′-carboxyrhodamine dyes lacking the 4′(5′)-carboxy substitutions permit a simplified synthesis of single isomer dyes but possess similar reactivities useful for labeling studies.
