77290-43-8

Upstream product

• 619-66-9 4-Carboxybenzaldehyde 
• 121-69-7 N,N-dimethyl-aniline 
• 34101-38-7 4-[bis(4-dimethylaminophenyl)methyl]benzoic acid methyl ester 
• 1571-08-0 methyl 4-formylbenzoate 
• 105-07-7 4-cyanobenzaldehyde 
• 861605-04-1 4-(4,4'-bis-dimethylamino-benzhydryl)-benzonitrile 

Downstream Products

• 6362-26-1 N-(4-((4-carboxyphenyl)(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-yliden)-N-methylmethanaminium chloride 
• 42297-54-1 N-(4-((4-carboxyphenyl)(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-methylmethanaminium 
• 229963-55-7 4-[bis-(4-dimethylamino-phenyl)-methyl]-benzoic acid 2,5-dioxo-pyrrolidin-1-yl ester 

Relevant academic research and scientific papers

Synthesis and cytotoxic evaluation of malachite green derived oleanolic and ursolic acid piperazineamides

The coupling of acetylated piperazinylamide spacered triterpenoic oleanolic acid and ursolic acid with meta or para substituted carboxylated malachite green analogs gave conjugates 10, 11, 15, and 16 that were cytotoxic for several human tumor cell lines. Especially, an oleanolic acid-derived compound 10 was cytotoxic for MCF-7 human breast carcinoma cells (EC50 = 0.7 μM). These derivatives represent first examples of triterpenoic acid derivatives holding a cationic scaffold derived from malachite green. [Figure not available: see fulltext.]

Malachite green artificial antigen and antibody and its preparation method and application

The invention provides preparation of malachite green artificial antigen and antibody, and relates to preparation of artificial hapten, artificial antigen and antibody of a triphenylmethane chemical substance malachite green. By adopting the preparation method, the problems of complex process, high cost and low analysis speed of the traditional physical and chemical analysis method are overcome. A simple, convenient, quick, sensitive and accurate enzyme immunoassay is provided. The method comprises the following steps: taking 4-[bi(4-dimethylamino)phenyl]methyl benzoic acid and 5-{4-[bi-(4-dimethylamino)phenyl]methyl} phenyl valeric acid as haptens to be respectively connected with carrier proteins such as hemocyanin, ovalbumin and the like to synthetize artificial antigen; carrying out animal immunization, taking blood, separating out antiserum, and purifying, so as to prepare the antibody. The antibody is stable, and has good specificity and sensitivity, and a good application prospect, and can be applied to fast immunodetection of the malachite green.

Production of antibodies for selective detection of malachite green and the related triphenylmethane dyes in fish and fishpond water

This study provides a practical method for production of the antibodies against malachite green (MG) and its primary metabolite leucomalachite green (LMG). Two ELISA kits are constructed with the MG and LMG antibodies for detection of the residual MG and LMG in fish muscle and fishpond water. The detection limit is established at the level of 0.05 μg/L for both MG and LMG. Our ELISA kits show the advantages of good specificity, high sensitivity, and convenience in rapid screening of MG and LMG residues. The sample of fishpond water, without extraction or prior preparation, is directly assayed by the ELISA kit. More then 80 fish samples can be simultaneously tested in a kit. The toxic crystal violet and its metabolite leucocrystal violet of illegal use in aquaculture are detected by our prepared MG and LMG antibodies, whereas the antibodies do not cross-react with common antibiotics, sulfonamides, and benzene derivatives.

Ip3 receptor ligands

A compound represented by the general formula (I) or a salt thereof, wherein R represents a group represented by the following formula (A), (B) or (C) wherein R1and R2independently represent a C1-6alkyl group, R3represents an amino group, a mono(C1-6alkyl)amino group, a di(C1-6alkyl)amino group or a C1-6alkoxy group and X-represents an anion, and n represents an integer of 2 to 5. The compound or a salt is useful as a ligand of inositol-1,4,5-trisphosphate (IP3) receptor.

Synthesis and evaluation of 1-position-modified inositol 1,4,5- trisphosphate analogs

IP3 analogs were synthesized by the modification of phosphate at the 1- position, and their affinity for the IP3 receptor was analyzed by means of surface plasmon resonance measurements. Our results suggest that a hydrophobic and cha