79259-43-1Relevant academic research and scientific papers
Peptide Synthesis by Means of Immobilized Enzymes. I. Immobilized α-Chimotripsin
Koennecke, Andreas,Bullerjahn, Ralf,Jakubke, Hans-Dieter
, p. 469 - 482 (2007/10/02)
α-Chymotrypsin covalently bound to silica, enzacryl AA, and enzacryl AH catalyzes peptide bond formation between N-protected dipeptide methyl esters and H-Leu-NH2 with results similar to those with the free enzyme.The influence of water-miscible and water
Model Studies on Papain-Catalyzed Peptide Synthesis in a Biphasic Aqueous-Organic System
Doering, Guenter,Kuhl, Peter,Jakubke, Hans-Dieter
, p. 1165 - 1174 (2007/10/02)
Several model peptides have been synthesized enzymatically using papain as a catalyst in biphasic aqueous-organic systems.The effect of different cosolvents, pH, buffer concentration, and reaction time on the papain-catalyzed synthesis was examined.A comp
Attempts for the Application of Thermitase as a Catalyst for Peptide Bond Formation
Koennecke, Andreas,Jakubke, Hans-Dieter
, p. 1099 - 1102 (2007/10/02)
The utility of thermitase, a thermophilic proteolytic enzyme from Thermoactinomyces vulgaris, as a catalyst for peptide synthesis has been studied using several Nα-benzyloxycarbonyl dipeptide esters as carboxyl components and Leu-NH2 as nucleophile.Couplings were most successfull with substrates containing Met, Leu, Ala, Phe, Tyr in P1-position, and Val, Ala, Pro in P2, whereas the substrates with Val, Ile, Pro in P1 failed to couple. - Keywords: Enzymatic peptide synthesis; Thermitase
α-Chymotrypsin-catalyzed Synthesis of Tripeptide Amides in an Aqueous-Organic Two-phase System
Kuhl, P.,Posselt, Siegrid,Jakubke, H.-D.
, p. 463 - 465 (2007/10/02)
α-Chymotrypsin catalyzes in a biphasic system containing an organic solvent and hydrogencarbonate buffer the reaction of Boc-Leu-Phe-OMe with X-NH2 (X=Leu, Met, Val) forming Boc-tripeptide amides.In the case of X=Leu the addition of ammoniumsulfate is advantageous and allows the use of carboxyl and amino component in equivalent amounts.By the same method Z-protected tripeptide amides can be prepared from Z-X-Phe-OMe (X=Ala, Leu, Val) and Leu-NH2 in good yields.In some case α-chymotrypsin-catalyzed peptide coupling is also possible, if the buffer solution is substituted for crystal water containing sodium carbonate.
