792965-89-0Relevant academic research and scientific papers
An efficient fluorescent probe for ratiometric pH measurements in aqueous solutions
Charier, Sandrine,Ruel, Odile,Baudin, Jean-Bernard,Alcor, Damien,Allemand, Jean-Francois,Meglio, Adrien,Jullien, Ludovic
, p. 4785 - 4788 (2004)
Just as litmus paper changes from red to blue with an increase in pH, so the fluorescence emission of the oxazole derivative 1 shifts from green to blue. Ratiometric measurements of the fluorescence emissions of the two species, after one- or two-photon e
Photophysics of a series of efficient fluorescent pH probes for dual-emission-wavelength measurements in aqueous solutions
Charier, Sandrine,Ruel, Odile,Baudin, Jean-Bernard,Alcor, Damien,Allemand, Jean-Francois,Meglio, Adrien,Jullien, Ludovic,Valeur, Bernard
, p. 1097 - 1113 (2007/10/03)
This paper evaluates the 5-aryl-2-pyridyloxazole backbone to engineer donor-acceptor fluorescent pH probes after one- or two-photon absorption. Parent fluorophores, as well as derivatives that can be used to label biomolecules, can be easily obtained in good yields. These molecules exhibit a large one-photon absorption in the near-UV range, and a strong fluorescence emission that covers the whole visible domain. The 5-aryl-2-pyridyloxazole derivatives also possess significant cross sections for two-photon absorption. Upon pyridine protonation, large shifts were observed in the absorption spectra after one- and two-photon excitation, as well as in the emission spectra. This feature was used to measure the pKa of the investigated compounds that range between 2 and 8. In most of the investigated derivatives, the pKa increased upon light excitation and protonation exchanges took place during the lifetime of the excited state, as shown by phase-modulation fluorometry analysis. Several 5-aryl-2-pyridyloxazole derivatives are suggested as efficient probes to reliably measure the pH of aqueous solutions by means of ratiometric methods that are dependent on fluorescence emission.
