800376-82-3 Usage
General Description
Phenylethyl-β-D-thiogalactopyranoside is a chemical compound used in biochemical research as a substrate for the analysis of β-galactosidase activity. It is a synthetic analog of lactose and is specifically designed to mimic the natural substrate for β-galactosidase, an enzyme involved in lactose metabolism. Phenylethylb-D-thiogalactopyranoside is often used as a colorimetric indicator in assays to detect the presence of β-galactosidase in cells or tissues. Upon hydrolysis by the enzyme, phenylethyl-β-D-thiogalactopyranoside releases a yellow product, which can be quantified spectrophotometrically to measure enzyme activity. Phenylethylb-D-thiogalactopyranoside is an important tool for studying the function and regulation of β-galactosidase in various biological systems.
Check Digit Verification of cas no
The CAS Registry Mumber 800376-82-3 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 8,0,0,3,7 and 6 respectively; the second part has 2 digits, 8 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 800376-82:
(8*8)+(7*0)+(6*0)+(5*3)+(4*7)+(3*6)+(2*8)+(1*2)=143
143 % 10 = 3
So 800376-82-3 is a valid CAS Registry Number.
InChI:InChI=1/C14H20O5S/c15-8-10-11(16)12(17)13(18)14(20-10)19-7-6-9-4-2-1-3-5-9/h1-5,10-18H,6-8H2/t10-,11-,12+,13-,14-/m1/s1
800376-82-3Relevant articles and documents
Probing the aglycon promiscuity of an engineered glycosyltransferase
Gantt, Richard W.,Goff, Randal D.,Williams, Gavin J.,Thorson, Jon S.
supporting information; experimental part, p. 8889 - 8892 (2009/05/26)
(Figure Presented) A sweet library: Two variants (wild-type (WT) and a triple mutant) of glycosyltransferase (GT) OleD have been shown to catalyze glycosylation of over 70 substrates, formation of O-, S- and N-glycosidic bonds, and iterative glycosylation (see scheme). Identified substrates include nucleophiles not previously known to act in GT reactions and span numerous natural product and therapeutic drug classes.
