800376-82-3 Usage
Uses
Used in Biochemical Research:
Phenylethyl-β-D-thiogalactopyranoside is used as a substrate for the analysis of β-galactosidase activity, allowing researchers to study the function and regulation of this enzyme in various biological systems.
Used in Colorimetric Assays:
Phenylethyl-β-D-thiogalactopyranoside is used as a colorimetric indicator in assays to detect the presence of β-galactosidase in cells or tissues. Upon hydrolysis by the enzyme, it releases a yellow product, which can be quantified spectrophotometrically to measure enzyme activity.
Used in Enzyme Activity Measurement:
Phenylethyl-β-D-thiogalactopyranoside is used for measuring β-galactosidase enzyme activity by providing a quantifiable yellow product upon hydrolysis. This allows for the assessment of enzyme activity levels in different samples, contributing to the understanding of enzyme function and regulation in biological systems.
Check Digit Verification of cas no
The CAS Registry Mumber 800376-82-3 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 8,0,0,3,7 and 6 respectively; the second part has 2 digits, 8 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 800376-82:
(8*8)+(7*0)+(6*0)+(5*3)+(4*7)+(3*6)+(2*8)+(1*2)=143
143 % 10 = 3
So 800376-82-3 is a valid CAS Registry Number.
InChI:InChI=1/C14H20O5S/c15-8-10-11(16)12(17)13(18)14(20-10)19-7-6-9-4-2-1-3-5-9/h1-5,10-18H,6-8H2/t10-,11-,12+,13-,14-/m1/s1
800376-82-3Relevant academic research and scientific papers
Probing the aglycon promiscuity of an engineered glycosyltransferase
Gantt, Richard W.,Goff, Randal D.,Williams, Gavin J.,Thorson, Jon S.
supporting information; experimental part, p. 8889 - 8892 (2009/05/26)
(Figure Presented) A sweet library: Two variants (wild-type (WT) and a triple mutant) of glycosyltransferase (GT) OleD have been shown to catalyze glycosylation of over 70 substrates, formation of O-, S- and N-glycosidic bonds, and iterative glycosylation (see scheme). Identified substrates include nucleophiles not previously known to act in GT reactions and span numerous natural product and therapeutic drug classes.
