80165-13-5Relevant articles and documents
Attempts for the Application of Thermitase as a Catalyst for Peptide Bond Formation
Koennecke, Andreas,Jakubke, Hans-Dieter
, p. 1099 - 1102 (1981)
The utility of thermitase, a thermophilic proteolytic enzyme from Thermoactinomyces vulgaris, as a catalyst for peptide synthesis has been studied using several Nα-benzyloxycarbonyl dipeptide esters as carboxyl components and Leu-NH2 as nucleophile.Couplings were most successfull with substrates containing Met, Leu, Ala, Phe, Tyr in P1-position, and Val, Ala, Pro in P2, whereas the substrates with Val, Ile, Pro in P1 failed to couple. - Keywords: Enzymatic peptide synthesis; Thermitase