80165-16-8Relevant academic research and scientific papers
Peptide Synthesis by Means of Immobilized Enzymes II. Immobilized Trypsin, Thermolysin and Papain
Koennecke, Andreas,Haensler, Marion,Schellenberger, Volker,Jakubke, Hans-Dieter
, p. 433 - 444 (2007/10/02)
Model studies were performed on the utility of covalently immobilized trypsin, thermolysin and papain for peptide bond formation.Trypsin and thermolysin catalyzed the formation of peptide bonds with nearly the same efficiency as the soluble proteases and they could be re-used successfully for further coupling experiments.The possibility of using immobilized trypsin and papain for kinetically controlled peptide bond formation was investigated.With the serine type enzyme trypsin excellent product yields were obtained starting with ester carboxyl components and an economical ratio of substrates.Experiments with the thiol protease papain were unsatisfactory because the once formed product is hydrolyzed as fact as the starting ester substrate used. - Keywords: Immobilized enzymes; Papain; Peptide synthesis; Thermolysin; Trypsin
Attempts for the Application of Thermitase as a Catalyst for Peptide Bond Formation
Koennecke, Andreas,Jakubke, Hans-Dieter
, p. 1099 - 1102 (2007/10/02)
The utility of thermitase, a thermophilic proteolytic enzyme from Thermoactinomyces vulgaris, as a catalyst for peptide synthesis has been studied using several Nα-benzyloxycarbonyl dipeptide esters as carboxyl components and Leu-NH2 as nucleophile.Couplings were most successfull with substrates containing Met, Leu, Ala, Phe, Tyr in P1-position, and Val, Ala, Pro in P2, whereas the substrates with Val, Ile, Pro in P1 failed to couple. - Keywords: Enzymatic peptide synthesis; Thermitase
