80220-63-9Relevant academic research and scientific papers
Glycan array on aluminum oxide-coated glass slides through phosphonate chemistry
Chang, Shih-Huang,Han, Jeng-Liang,Tseng, Susan Y.,Lee, Hsin-Yu,Lin, Chin-Wei,Lin, Yu-Chen,Jeng, Wen-Yih,Wang, Andrew H.-J.,Wu, Chung-Yi,Wong, Chi-Huey
supporting information; experimental part, p. 13371 - 13380 (2010/11/18)
A new type of glycan array covalently or noncovalently attached to aluminum oxide-coated glass (ACG) slides has been developed for studies of enzymatic reactions and protein binding. To prepare the noncovalent array, glycans with a polyfluorinated hydrocarbon (-C8F17) tail are spotted robotically onto the ACG slide surface containing a layer of polyfluorinated hydrocarbon terminated with phosphonate. After incubation and washing, the noncovalent array can be characterized by MS-TOF via ionization/desorption at a low laser energy without addition of matrix. A representive cellotetraose array was developed to study the activity and specificity of different cellulases and to differentiate the exo-and endoglucanase activities. To prepare the covalent array, glycans with a phosphonic acid tail were synthesized and spotted robotically onto the ACG slide surface. After incubation, the slides can be used directly for quantitative protein binding analysis. Compared to the preparation of glycan arrays on glass slides and other surfaces, this method of arraying using phosphonic acid reacting with ACG is more direct, convenient, and effective and represents a new platform for the high-throughput analysis of protein-glycan interactions.
FLUORINATED PHOSPHONIC ACIDS
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Page 7, (2010/02/05)
Fluorinated phosphonic acid compounds, useful as treatments for substrate surfaces, have the formula (I) : wherein: R?1? is a straight chain alkylene group having from 3 to 21 carbon atoms, an oxa-substituted straight chain alkylene group having from 2 to 20 carbon atoms, or a thia-substituted straight chain alkylene group having from 2 to 20 carbon atoms; R?2? is a perfluoroalkyl group having from 4 to 10 carbon atoms; R?3? is hydrogen, an alkali metal cation, or an alkyl group having from 1 to 6 carbon atoms; and M is hydrogen or an alkali metal cation, with the proviso that if R?1? is an unsubstituted straight chain alkylene group, then the sum of carbon atoms in R?1? and R?2? combined is at least 10.
