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CAS

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80395-90-0

ALKALINE PHOSPHATASE STOP SOLUTION is a specialized reagent designed to terminate the enzymatic activity of alkaline phosphatase in various biological assays. It is crucial for stopping the reaction involving alkaline phosphatase and its substrates, such as nitrotetrazolium blue chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP), to prevent further color development and ensure accurate results.

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  • Phosphatase,alkaline (Escherichia coli precursor signal peptide)

    Cas No: 80395-90-0

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  • 80395-90-0 Structure

  • Basic information

    1. Product Name: ALKALINE PHOSPHATASE STOP SOLUTION
    2. Synonyms: ALKALINE PHOSPHATASE STOP SOLUTION;ALKALINE PHOSPHATASE
    3. CAS NO:80395-90-0
    4. Molecular Formula: C101H173N23O26
    5. Molecular Weight: 2125.59
    6. EINECS: N/A
    7. Product Categories: Alkaline Phosphatase;Alphabetical ListingELISA Substrates;Detection Substrates;Enzyme Substrates;Liquid Substrate Systems
    8. Mol File: 80395-90-0.mol

  • Chemical Properties

    1. Melting Point: N/A
    2. Boiling Point: N/A
    3. Flash Point: N/A
    4. Appearance: /
    5. Density: N/A
    6. Refractive Index: N/A
    7. Storage Temp.: 2-8°C
    8. Solubility: N/A
    9. CAS DataBase Reference: ALKALINE PHOSPHATASE STOP SOLUTION(CAS DataBase Reference)
    10. NIST Chemistry Reference: ALKALINE PHOSPHATASE STOP SOLUTION(80395-90-0)
    11. EPA Substance Registry System: ALKALINE PHOSPHATASE STOP SOLUTION(80395-90-0)

  • Safety Data

    1. Hazard Codes: Xi
    2. Statements: 36/37/38
    3. Safety Statements: 26-36
    4. WGK Germany: 3
    5. RTECS:
    6. HazardClass: N/A
    7. PackingGroup: N/A
    8. Hazardous Substances Data: 80395-90-0(Hazardous Substances Data)

80395-90-0 Usage

Uses

Used in Molecular Biology and Diagnostics Industry:
ALKALINE PHOSPHATASE STOP SOLUTION is used as a reaction terminator for stopping the alkaline phosphatase and nitrotetrazolium blue chloride (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) reaction on nitrocellulose membrane. This application is essential for accurate colorimetric assays and ensures reliable results in various diagnostic and research applications.
Used in Colorimetric Assays:
ALKALINE PHOSPHATASE STOP SOLUTION is used as a stop solution for Alkaline Phosphatase Red and Blue microwell substrate solutions. It is applied by using 50 μl of stop solution per 200 μl of reaction mix, effectively halting the enzymatic activity and preventing further color development in the assay. This precise control over the reaction is vital for obtaining accurate and reproducible results in colorimetric assays.

Check Digit Verification of cas no

The CAS Registry Mumber 80395-90-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,0,3,9 and 5 respectively; the second part has 2 digits, 9 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 80395-90:
(7*8)+(6*0)+(5*3)+(4*9)+(3*5)+(2*9)+(1*0)=140
140 % 10 = 0
So 80395-90-0 is a valid CAS Registry Number.

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