Valyl-glycyl-arginine-4-nitroanilide (VGA-4NA) is a synthetic substrate commonly used in biochemical research to study the activity of proteolytic enzymes, particularly trypsin and other serine proteases. This substrate consists of a peptide sequence (valine-glycine-arginine) linked to a chromogenic moiety, 4-nitroanilide. Upon enzymatic cleavage, the 4-nitroanilide group is released, resulting in a color change that can be quantified spectrophotometrically, allowing for the assessment of enzyme activity and kinetics. VGA-4NA is widely employed in laboratories for the characterization of proteases, drug screening, and the development of inhibitors targeting these enzymes.
80798-23-8
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Basic information
- Product Name: valyl-glycyl-arginine-4-nitroanilide
- Synonyms: valyl-glycyl-arginine-4-nitroanilide
- CAS NO:80798-23-8
- Molecular Formula: C19H30 N8 O5
- Molecular Weight: 450.49
- EINECS: N/A
- Product Categories: N/A
- Mol File: 80798-23-8.mol
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: 1.43g/cm3
- Refractive Index: 1.64
- Storage Temp.: N/A
- Solubility: N/A
- CAS DataBase Reference: valyl-glycyl-arginine-4-nitroanilide(CAS DataBase Reference)
- NIST Chemistry Reference: valyl-glycyl-arginine-4-nitroanilide(80798-23-8)
- EPA Substance Registry System: valyl-glycyl-arginine-4-nitroanilide(80798-23-8)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany:
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 80798-23-8(Hazardous Substances Data)
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Check Digit Verification of cas no
The CAS Registry Mumber 80798-23-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,0,7,9 and 8 respectively; the second part has 2 digits, 2 and 3 respectively.Calculate Digit Verification of CAS Registry Number 80798-23:
(7*8)+(6*0)+(5*7)+(4*9)+(3*8)+(2*2)+(1*3)=158
158 % 10 = 8
So 80798-23-8 is a valid CAS Registry Number.
InChI:InChI=1/C19H30N8O5/c1-11(2)16(20)18(30)24-10-15(28)26-17(29)14(4-3-9-23-19(21)22)25-12-5-7-13(8-6-12)27(31)32/h5-8,11,14,16,25H,3-4,9-10,20H2,1-2H3,(H,24,30)(H4,21,22,23)(H,26,28,29)/t14-,16+/m0/s1
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valyl-glycyl-arginine-4-nitroanilide
