81-93-6 Usage
Uses
Different sources of media describe the Uses of 81-93-6 differently. You can refer to the following data:
1. Phenosafranin is a bacterial stain used in microscopy.
2. Phenosafranin has been used for nuclear staining.
Definition
ChEBI: An organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms.
Biochem/physiol Actions
Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.
Purification Methods
Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]
Check Digit Verification of cas no
The CAS Registry Mumber 81-93-6 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 8 and 1 respectively; the second part has 2 digits, 9 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 81-93:
(4*8)+(3*1)+(2*9)+(1*3)=56
56 % 10 = 6
So 81-93-6 is a valid CAS Registry Number.
InChI:InChI=1/C18H14N4.ClH/c19-12-6-8-15-17(10-12)22(14-4-2-1-3-5-14)18-11-13(20)7-9-16(18)21-15;/h1-11H,(H3,19,20);1H
81-93-6Relevant articles and documents
1-(Phenylamino)pseudo-mauveine: A new water soluble phenazine dye
Plater, M. John,Jackson, Toby
, p. 437 - 442 (2014/08/05)
1-(Phenylamino)pseudo-mauveine was prepared by the oxidation of 1,3,5-tris(phenylamino)benzene and p-phenylenediamine with potassium dichromate. The absorption maximum of 535 nm is shifted hypsochromically by about 20 nm compared to that for pseudo-mauvei
Acid-base catalyzed photoinduced redox reactions of phenosafranin-EDTA system in photoelectrochemical cell
Jana, Asok K.,Bhowmik, Benoy B.
, p. 351 - 355 (2007/10/02)
The photogalvanic effect in phenosafranin-EDTA aqueous system has been studied at different pH.The photovoltage growth and decay curves follow the functional forms related to the relaxation times.Under the experimental conditions, the inverse of relaxation time or the rate of electrode reaction is pH-dependent.The rate constants (k) of forward and backward reactions at 25 deg C have been calculated.From the plot of log k versus pH, catalytic constants for H(+) and OH(-) have been determined.The calculated free energy of electron transfer across the electrode/electrolyte interface is about -5.66 kJ mol-1.
