81239-45-4

Usage

Uses

Used in Biochemistry and Molecular Biology:
NDSB-256 is used as a solubilizing and stabilizing agent for proteins to improve their renaturation and reduce aggregation. It is particularly useful for restoring enzymatic activity in denatured proteins, such as chicken egg white lysozyme and E. coli β-galactosidase.
At a concentration of 1M, NDSB-256 can restore 30% of the enzymatic activity of denatured egg white lysozyme, while at 800mM, it can restore 16% of the enzymatic activity of denatured β-galactosidase. This makes it a valuable tool in protein refolding and stabilization for research and industrial applications.

InChI:InChI=1/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3

Upstream product

• 1120-71-4 1,3-propanesultone 
• 103-83-3 N,N'-dimethylbenzylamine 

Relevant academic research and scientific papers

Construction and application of 2, 6-dibromopyridine oxidation system

The invention discloses a construction method of a 2, 6-dibromopyridine oxidation system. The construction method comprises the following steps: using 2, 6-dibromopyridine as a reaction substrate, andorming an acidic ionic liquid and 2, 6-dibromopyridine

Method for preparing advanced fatty acid ester by using catalysis of solid-supported ionic liquid catalyst

The invention discloses a method for preparing fatty acid ester by using catalysis of a solid-supported ionic liquid catalyst. The method comprises the following steps of (1) by taking fatty acid and alcohol as raw materials and the solid-supported ionic liquid catalyst as a catalyst, reacting in an oil bath of 60-170 DEG C for 0.5-6 hours; and (2) cooling after reaction is completed, layering the catalyst and a reaction system, carrying out suction filtration on a product to separate a solid catalyst, washing through diethyl ether and drying for direct next reaction. Excessive alcohol is evaporated, and the advanced fatty acid ester and glycerel monostearte are obtained through reduced pressure distillation. The fatty acid ester prepared in the step (1) preferably reacts in the oil bath of 90 DEG C for 2 hours. Fatty acid monoglyceride preferably reacts in the oil bath of 140 DEG C for 45 minutes. The yield and purity of the fatty acid ester prepared through catalysis under the combination of preferred conditions can reach higher values.

Synthesis of quaternary alkylammonium sulfobetaines

A series of quaternary alkylammonium sulfobetaines of general formula RN+(CH3)2(CH2)nSO 3-, where n = 2-4, have been synthesised by reacting the corresponding N,N-dimethylamines with either sodium 2-chloroethanesulfonate (n = 2), 1,3-propane-sultone (n = 3), or 1,4-butanesultone (n = 4). Full details of the preparation of the required N,N-dimethylamines are reported.