BamH I is a type IIS restriction endonuclease that recognizes the specific DNA sequence G↓GATCC and cuts the DNA, generating fragments with 5′-cohesive termini. It is an enzyme commonly used in molecular biology and genetic engineering for DNA manipulation and analysis.
81295-09-2
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Basic information
- Product Name: BAMH I
- Synonyms: endonucleasebamhi;restrictionendodeoxyribo-nucleasbamhi;restrictionendonucleasealii;restrictionendonucleasebamhi;BAMH I;BAMH I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASES BAMH I;restriction endonuclease bamh I from*bacillus
- CAS NO:81295-09-2
- Molecular Formula:
- Molecular Weight: 0
- EINECS: 279-720-4
- Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: Store at -20
- Solubility: N/A
- CAS DataBase Reference: BAMH I(CAS DataBase Reference)
- NIST Chemistry Reference: BAMH I(81295-09-2)
- EPA Substance Registry System: BAMH I(81295-09-2)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany:
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 81295-09-2(Hazardous Substances Data)
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81295-09-2 Usage
Uses
Used in Genetic Engineering:BamH I is used as a restriction enzyme for cloning and genetic engineering applications. It is employed to cut DNA at specific recognition sites, allowing for the insertion or manipulation of genes within a DNA molecule.
Used in DNA Fragmentation:
BamH I is used as a tool for DNA fragmentation in various molecular biology techniques. The enzyme generates fragments with 5′-cohesive termini, which can be easily ligated to other DNA fragments, facilitating the assembly of recombinant DNA molecules.
Used in DNA Sequencing:
BamH I is used as a sequencing enzyme in some DNA sequencing methods. The generated 5′-cohesive termini allow for the efficient cloning of DNA fragments, which can then be sequenced to determine their nucleotide composition.
Used in Research and Diagnostics:
BamH I is used as a research and diagnostic tool in various fields, such as molecular biology, genetics, and biotechnology. The enzyme's ability to cut DNA at specific sites makes it a valuable resource for studying gene function, regulation, and expression, as well as for developing diagnostic tests and assays.
Check Digit Verification of cas no
The CAS Registry Mumber 81295-09-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,1,2,9 and 5 respectively; the second part has 2 digits, 0 and 9 respectively.Calculate Digit Verification of CAS Registry Number 81295-09:
(7*8)+(6*1)+(5*2)+(4*9)+(3*5)+(2*0)+(1*9)=132
132 % 10 = 2
So 81295-09-2 is a valid CAS Registry Number.
81295-09-2Upstream product
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BAMH I
