81520-71-0Relevant academic research and scientific papers
Ring-opening glycosylation of a chitobiose oxazoline catalyzed by a non-chitinolytic mutant of chitinase
Sakamoto, Junji,Watanabe, Takeshi,Ariga, Yumiko,Kobayashi, Shiro
, p. 1180 - 1181 (2001)
Ring-opening glycosylation of a chitobiose oxazoline was exclusively achieved by catalysis of a mutated chitinase that is only active for the glycosylation and not for chitinolysis.
Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC
Umemoto, Naoyuki,Ohnuma, Takayuki,Mizuhara, Mamiko,Sato, Hirokazu,Skriver, Karen,Fukamizo, Tamo
, p. 81 - 90 (2013/01/16)
A tryptophan side chain was introduced into subsite +1 of family GH-18 (class V) chitinases from Nicotiana tabacum and Arabidopsis thaliana (NtChiV and AtChiC, respectively) by the mutation of a glycine residue to tryptophan (G74W-NtChiV and G75W-AtChiC). The specific activity toward glycol chitin of the two mutant enzymes was 70-71% of that of the wild type. Using chitin oligosaccharides, (GlcNAc)n (n = 4, 5 and 6), as the substrates, we found the transglycosylation reaction to be significantly enhanced in G74W-NtChiV and G75W-AtChiC when compared with the corresponding wild-type enzymes. The introduced tryptophan side chain might protect the oxazolinium ion intermediate from attack by a nucleophilic water molecule. The enhancement of transglycosylation activity was much more distinct in G75W-AtChiC than in G74W-NtChiV. Nuclear magnetic resonance titration experiments using the inactive double mutants, E115Q/G74W-NtChiV and E116Q/G75W-AtChiC revealed that the association constant of (GlcNAc)5 was considerably larger for the latter. Amino acid substitutions at the acceptor binding site might have resulted in the larger association constant for G75W-AtChiC, giving rise to the higher transglycosylation activity of G75W-AtChiC. The Author 2012. Published by Oxford University Press. All rights reserved.
