815591-73-2Relevant academic research and scientific papers
A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems
Agard, Nicholas J.,Prescher, Jennifer A.,Bertozzi, Carolyn R.
, p. 15046 - 15047 (2004)
Selective chemical reactions that are orthogonal to the diverse functionality of biological systems have become important tools in the field of chemical biology. Two notable examples are the Staudinger ligation of azides and phosphines and the Cu(I)-catal
Compositions and methods for modification of biomolecules
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, (2016/03/12)
The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provide for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).
Application of strain-promoted azide-alkyne cycloaddition and tetrazine ligation to targeted fc-drug conjugates
Thomas, Joshua D.,Cui, Huiting,North, Patrick J.,Hofer, Thomas,Rader, Christoph,Burke, Terrence R.
, p. 2007 - 2013 (2013/01/15)
We have previously described an approach whereby antibody Fc fragments harboring a single C-terminal selenocysteine residue (Fc-Sec) are directed against a variety of targets by changing the peptide or small molecule to which they are conjugated. In the p
