81969-80-4Relevant articles and documents
Structure-Guided Directed Evolution of a Carbonyl Reductase Enables the Stereoselective Synthesis of (2 S,3 S)-2,2-Disubstituted-3-hydroxycyclopentanones via Desymmetric Reduction
Li, Juan,Feng, Jinhui,Chen, Xi,Gong, Jingyao,Cui, Yunfeng,Zhang, Hongliu,Bu, Dandan,Wu, Qiaqing,Zhu, Dunming
, p. 3444 - 3448 (2020)
In this study, an engineered carbonyl reductase (M4) was obtained through structure-guided directed evolution of a carbonyl reductase (SSCR) from Sporobolomyces salmonicolor AKU4429. Mutant M4 showed 23.9-fold enhancement of enzyme activity toward the model substrate 2-methyl-2-benzyl-1,3-cyclopentanedione, affording the (2S,3S)-stereoisomer in >98percent ratio. This variant also showed excellent stereoselectivity toward most of the tested substrates, offering a valuable biocatalyst for the stereoselective reduction of these cyclic diketones to access the corresponding (2S,3S)-2,2-disubstituted-3-hydroxyketones.
Highly stereocontrolled reduction of 1,3-cyclopentanediones using oxazaborolidine -BH3
Shimizu, Makoto,Yamada, Shunsuke,Fujita, Yoshifumi,Kobayashi, Fumiko
, p. 3883 - 3886 (2007/10/03)
Highly enantioselective reduction of 1,3-cyclopentanediones was conducted using an oxazaborolidine derived from L-threonine and a borane complex to give either 1,3-cyclopentanediols or 3-hydroxycyclopentanones in high enantiomeric purity by choosing appropriate reduction conditions. Copyright (C) 2000 Elsevier Science Ltd.
