82120-92-1Relevant academic research and scientific papers
Improved LC-MS method for the determination of fatty acids in red blood cells by LC-orbitrap MS
Li, Xingnan,Franke, Adrian A.
, p. 3192 - 3198 (2011/11/04)
We report a new method for fast and sensitive analyses of biologically relevant fatty acids (FAs) in red blood cells (RBC) by liquid chromatography mass spectrometry (LC-MS). A new chemical derivatization approach was developed forming picolylamides from FAs in a quantitative reaction. Fourteen derivatized FA standards, including saturated and unsaturated FAs from C14 to C22, were efficiently separated within 15 min. In addition, the use of a recently introduced benchtop orbitrap mass spectrometer under positive electrospray ionization (ESI) full scan mode showed a 2-10-fold improvement in sensitivity compared with a conventional tandem MS method, with a limit of detection in the low femtomole range for saturated and unsaturated FAs. The developed method was applied to determine FA concentrations in RBC with intra- and interday coefficients of variation below 10%.
Triple Quadrupole Tandem Mass Spectrometric Study of the 3-Picolinyl Esters of Fatty Acids
Rubino, Federico Maria,Zecca, Luigi
, p. 1240 - 1247 (2007/10/02)
Low-energy collision-induced dissociation of some representative 3-picolinyl esters of fatty acids were investigated.The daughter-ion spectra of the precursor ions showed similar features to the electron impact mass spectra, but the low intensity of the parent-ion signal did not allow reliable deductions.The parent-ion spectra of some fragments that retain the pyridine derivatizing group contain structurally useful information, and are suitable for analytical application.These spectra also contain fragment ions which may account for an alternative pathway to thatcurrently proposed for the generation of certain fragment ions within the ion source.Both pathways were investigated in this tandem mass spectrometric study.Isomerization and decomposition of distonic fragment ions through loss of alkenes yield species with a shorter alkyl chain, which are particularly stable when they contain allylic radicals.
