82954-73-2Relevant academic research and scientific papers
Incorporation of spin-labelled amino acids into proteins
Becker, Christian F. W.,Lausecker, Kester,Balog, Maria,Kalai, Tamas,Hideg, Kalman,Steinhoff, Heinz-Juergen,Engelhard, Martin
, p. S34-S39 (2005)
The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins. Copyright
Synthesis of new 2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-1-yloxyl radicals and 2-substituted-2,5,5-trimethylpyrrolidin-1-yloxyl radicals based α-amino acids
Balog, Mária,Kálai, Tamás,Jeko, József,Steinhoff, Heinz-Jürgen,Engelhard, Martin,Hideg, Kálmán
, p. 2591 - 2593 (2007/10/03)
Unnatural paramagnetic α-amino acids with 2,2,5,5-tetramethyl-2,5- dihydro-1H-pyrrol-1-yloxyl-3-yl radical or 2,5,5-trimethylpyrrolidin-1-yloxyl-2- yl radical side-chains, including a lysine mimic azido precursor and their derivatives, are described. The
Nitroxides. IX1 Synthesis of nitroxide free radical α-amino acids
Lex, Laszlo,Hideg, Kalman,Hankovszky, H. Olga
, p. 1448 - 1451 (2007/10/02)
Three methods are described for preparation of a new stable nitroxide free radical amino acid, 2-amino-3-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolin-3-yl) propionic acid and its derivatives.This amino acid may be used as a paramagnetic amino acid synthon in studies of peptides.
