83258-72-4Relevant academic research and scientific papers
Fluorometric Determination of Secondary Amines Based on Their Reaction with Fluorescamine
Nakamura, Hiroshi,Tamura, Zenzo
, p. 2087 - 2092 (2007/10/02)
A spectrophotofluorometric method has been developed for the determination of secondary amines by using fluorescamine.When compounds having a secondary amino group were reacted with fluorescamine at pH 12 and then heated at 70 deg C for 10 min with a primary amine (L-Leu-L-Ala), they gave a bluish green fluorescence.By measurement of the fluorescence, most secondary amines could be determined in 0.5 nmol quantities.Relative standard deviations of 4.4 and 5.2percent are observed for the analyses of 5 nmol of N-methylaniline and sarcosine, respectively.
