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Usage

Uses

Used in Tissue Culture Applications:
Serum Spreading Factor is used as a constituent of serum-free media for culturing skeletal muscle and for the growth and myelination of motoneurons by Schwann cells. It provides essential attachment and survival factors for these cells, promoting their proper development and function.
Used in Neuronal Research:
In the field of neuronal research, Serum Spreading Factor is used as a constituent of enriched co-culture media for culturing rat spinal cord. Its presence in the media helps support the growth and maintenance of neural cells, making it an important tool for studying neural development and function.
Used in Cancer Research:
Serum Spreading Factor has been used to study the effects of extracellular matrix (ECM) ligands on C6 astroglioma cells. Understanding the interactions between these cells and the ECM can provide valuable insights into the mechanisms of cancer progression and potential therapeutic targets.
Used as a Cell Culture Substratum:
Serum Spreading Factor can be used with cells that have integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at a concentration of 0.1 μg/cm2. The optimal conditions for attachment depend on the specific cell line and application, making Serum Spreading Factor a versatile tool for various cell culture experiments.

Biochem/physiol Actions

The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney.