84325-23-5 Usage
Description
4-Methylumbelliferyl-β-D-lactoside, with the chemical abstracts service number 84325-23-5, is a fluorescent coumarin glycoside. It is characterized by its ability to emit fluorescence, which makes it a valuable tool in various analytical and research applications.
Uses
Used in Enzymatic Assays:
4-Methylumbelliferyl-β-D-lactoside is utilized as a chromophoric substrate for enzymes such as cellobiohydrolase I (CBHI) in Trichoderma reesei, cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B), and β-glucosidase. Its use in these enzymatic assays is due to its fluorescent properties, which allow for the easy detection and measurement of enzymatic activity.
Used in the Preparation of Fluorescent Probes:
4-METHYLUMBELLIFERYL-BETA-D-LACTOSIDE is also used in the preparation of fluorescent probes specifically designed for sialidases and sialyltransferases. The reason for its use in this context is its ability to bind to these enzymes and fluoresce upon interaction, providing a sensitive and specific method for detecting and studying the activity of these enzymes.
Biochem/physiol Actions
4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1
Check Digit Verification of cas no
The CAS Registry Mumber 84325-23-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,4,3,2 and 5 respectively; the second part has 2 digits, 2 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 84325-23:
(7*8)+(6*4)+(5*3)+(4*2)+(3*5)+(2*2)+(1*3)=125
125 % 10 = 5
So 84325-23-5 is a valid CAS Registry Number.
InChI:InChI=1/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1
84325-23-5Relevant articles and documents
A multifunctional Pasteurella multocida sialyltransferase: A powerful tool for the synthesis of sialoside libraries
Yu, Hai,Chokhawala, Harshal,Karpel, Rebekah,Yu, Hui,Wu, Bingyuan,Zhang, Jianbo,Zhang, Yingxin,Jia, Qiang,Chen, Xi
, p. 17618 - 17619 (2005)
A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein