84522-15-6 Usage
Uses
Used in Clinical and Biochemical Laboratories:
Naphthol AS-OL phosphate is used as a diagnostic tool for detecting and monitoring various health conditions, such as liver and bone diseases. It helps in assessing the effectiveness of treatments by measuring the activity of alkaline phosphatase, an enzyme linked to these conditions.
Used in Histochemical Techniques:
In the field of histochemistry, naphthol AS-OL phosphate is employed as a staining reagent for the localization of phosphatase enzymes in tissues. This application aids researchers and pathologists in studying the distribution and activity of these enzymes within biological samples, contributing to a better understanding of their roles in health and disease.
Check Digit Verification of cas no
The CAS Registry Mumber 84522-15-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,4,5,2 and 2 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 84522-15:
(7*8)+(6*4)+(5*5)+(4*2)+(3*2)+(2*1)+(1*5)=126
126 % 10 = 6
So 84522-15-6 is a valid CAS Registry Number.
InChI:InChI=1/C18H16NO6P/c1-24-16-9-5-4-8-15(16)19-18(20)14-10-12-6-2-3-7-13(12)11-17(14)25-26(21,22)23/h2-11H,1H3,(H,19,20)(H2,21,22,23)
84522-15-6Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
