853014-42-3Relevant articles and documents
Design, synthesis, and biological evaluation of novel C14-C3′BzN- linked macrocyclic taxoids
Sun, Liang,Geng, Xudong,Geney, Raphael,Li, Yuan,Simmerling, Carlos,Li, Zhong,Lauher, Joseph W.,Xia, Shujun,Horwitz, Susan B.,Veith, Jean M.,Pera, Paula,Bernacki, Ralph J.,Ojima, Iwao
experimental part, p. 9584 - 9593 (2009/04/06)
(Figure Presented) Novel macrocyclic paclitaxel congeners were designed to mimic the bioactive conformation of paclitaxel. Computational analysis of the REDOR-Taxol structure revealed that this structure could be rigidified by connecting the C14 position of the baccatin moiety and the ortho position of C3′N-benzoyl group (C3′BzN), which are ca. 7.5 A apart, with a short linker (4-6 atoms). 7-TES-14β-allyloxybaccatin III and (3R,4S)-1-(2-alkenylbenzoyl)-β-lactams were selected as key components, and the Ojima-Holton coupling afforded the corresponding paclitaxel-dienes. The Ru-catalyzed ring-closing metathesis (RCM) of paclitaxel-dienes gave the designed 15- and 16-membered macrocyclic taxoids. However, the RCM reaction to form the designed 14-membered macrocyclic taxoid did not proceed as planned. Instead, the attempted RCM reaction led to the occurrence of an unprecedented novel Ru-catalyzed diene-coupling process, giving the corresponding 15-membered macrocyclic taxoid (SB-T-2054). The biological activities of the novel macrocyclic taxoids were evaluated by tumor cell growth inhibition (i.e., cytotoxicity) and tubulin-polymerization assays. Those assays revealed high sensitivity of cytotoxicity to subtle conformational changes. Among the novel macrocyclic taxoids evaluated, SB-T-2054 is the most active compound, which possesses virtually the same potency as that of paclitaxel. The result may also indicate that SB-T-2054 structure is an excellent mimic of the bioactive conformation of paclitaxel. Computational analysis for the observed structure-activity relationships is also performed and discussed.
