853799-71-0Relevant academic research and scientific papers
Preparation method of thieno[3, 4-b]-1, 4-dioxin-2-methanol derivative
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Paragraph 0038; 0046-0056; 0064-0074; 0082-0091, (2020/06/20)
The invention discloses a preparation method of a thieno[3, 4-b]-1, 4-dioxin-2-methanol derivative, which comprises the following steps: S1. adding a first reaction solvent, a raw material thieno[3, 4-b]-1, 4-dioxin-2-methanol, a first reactant and a catalyst into a three-neck flask to obtain a first mixed solution; S2, dropwise adding an alkaline solution; S3, reacting to obtain a first reactionsolution; S4, performing standing and layering on the first reaction solution to obtain an organic phase and an extracting solution; S5, combining the organic phase with the extracting solution, and drying; S6, filtering and drying to obtain an intermediate product; S7, adding a second reaction solvent and the intermediate product into the three-neck flask to obtain a mixed solution; S8, concentrating the mixed solution; S9, dropwise adding an alkaline solution; S10, extracting and collecting an organic phase; S11, washing the organic phase with water; S12, drying and concentrating the organicphase to obtain a second mixture; and S13, eluting the second mixture, and drying to obtain a target product.
Characterization of PEDOT film functionalized with a series of automated synthesis ferrocenyl-containing oligonucleotides
Navarro, Aude-Emmanuelle,Fages, Frédéric,Moustrou, Corinne,Brisset, Hugues,Spinelli, Nicolas,Chaix, Carole,Mandrand, Bernard
, p. 3947 - 3952 (2007/10/03)
In previous works we have described a fully automated synthesis of new ferrocene labelled oligonucleotides (Fc-ODNs) probes with one or more electroactive markers at different position in the chain. These Fc-ODNs have shown good properties to detect ODN target in solution. Here we describe the post-functionalization of a conducting co-polymer based on ethylenedioxythiophene (EDOT) derivatives by a series of Fc-ODNs. The grafting of the Fc-ODNs probes resulted in the appearance of the ferrocene redox couple which directly confirm the effectiveness of the ODN anchoring compared to traditional approach based on IR spectroscopy and X-ray fluorescence of the films. Moreover, the electrochemical response of the modified electrodes analysed in organic media before and after hybridization with ODN target confirm that properties obtain in solution for Fc-ODNs already exist in the film. The changes in the current intensity were found to be dependant on the structure of the grafted ODN that validate our strategy to synthesize an optimal Fc-ODNs.
