85597-60-0

Usage

Uses

Used in Molecular Biology Research:
Polythymidylic acid sodium salt is used as a research tool for studying the characteristics of single-stranded DNA binding proteins. It provides a specific and uniform substrate for investigating the interactions between proteins and single-stranded DNA, which is crucial for understanding the mechanisms of DNA replication, repair, and transcription.
Used in Nucleic Acid Hybridization:
Poly(dT) is utilized in nucleic acid hybridization techniques, such as Northern blotting and microarray analysis, to facilitate the annealing of complementary RNA sequences. Its high affinity for adenine-rich sequences allows for efficient and specific hybridization, enabling the detection and quantification of target RNA molecules.
Used in siRNA and miRNA Research:
Polythymidylic acid sodium salt is employed in the study of small non-coding RNA molecules, such as small interfering RNA (siRNA) and microRNA (miRNA). It serves as a template for the synthesis of RNA molecules with specific sequences, allowing researchers to investigate the roles of these regulatory RNAs in gene expression and cellular processes.
Used in Drug Delivery Systems:
Poly(dT) has potential applications in drug delivery systems, particularly for the development of nucleic acid-based therapeutics. Its ability to form stable complexes with other molecules, such as drugs or nanoparticles, can enhance the delivery, bioavailability, and therapeutic efficacy of various pharmaceutical agents.
Used in Nanotechnology and Biomaterials:
Polythymidylic acid sodium salt is utilized in the design and fabrication of nanostructures and biomaterials for various applications, including biosensors, diagnostics, and therapeutics. Its unique properties, such as its ability to form triple helix structures and its compatibility with other materials, make it a promising candidate for the development of innovative nanotechnological solutions.

Upstream product

• 77825-23-1 Phosphoric acid (2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-tetrahydro-furan-2-ylmethyl ester bis-(2,2,2-trichloro-1,1-dimethyl-ethyl) ester 
• 365-07-1 thymidine 5'-phosphate 
• 1608113-92-3 O,O-dibenzyl O-(3'-O-(tert-butyldimethylsilyl)-5'-deoxythymidine-5'-yl)phosphate 
• 5115-07-1 O,O-dibenzyl O-(5'-deoxythymidine-5'-yl)phosphate 

Downstream Products

• 61-19-8 5'-adenosine monophosphate 
• 365-07-1 thymidine 5'-phosphate 

Relevant academic research and scientific papers

5’-Phosphorylation Increases the Efficacy of Nucleoside Inhibitors of the DNA Repair Enzyme SNM1A

Certain cancers exhibit upregulation of DNA interstrand crosslink repair pathways, which contributes to resistance to crosslinking chemotherapy drugs and poor prognoses. Inhibition of enzymes implicated in interstrand crosslink repair is therefore a promising strategy for improving the efficacy of cancer treatment. One such target enzyme is SNM1A, a zinc co-ordinating 5’–3’ exonuclease. Previous studies have demonstrated the feasibility of inhibiting SNM1A using modified nucleosides appended with zinc-binding groups. In this work, we sought to develop more effective SNM1A inhibitors by exploiting interactions with the phosphate-binding pocket adjacent to the enzyme's active site, in addition to the catalytic zinc ions. A series of nucleoside derivatives bearing phosphate moieties at the 5’-position, as well as zinc-binding groups at the 3’-position, were prepared and tested in gel-electrophoresis and real-time fluorescence assays. As well as investigating novel zinc-binding groups, we found that incorporation of a 5’-phosphate dramatically increased the potency of the inhibitors.

Synthesis of 3'O-(2-cyanoethyl)-2'-deoxythymidine-5'-phosphate as a model compound for evaluation of Cyanoethyl Cleavage

An essential and challenging task during the development of our sequencing-by-synthesis (SBS) technique is the evaluation of efficient cyanoethyl (CE) cleavage conditions. For this purpose 3'-O-(2-cyanoethyl)-2'- deoxythymidine-5'-phosphate as a model compound as well as a short DNA oligomer bearing the CE function as terminal group were synthesized and used for various deprotection experiments. As it is already known for 2'-O-CE-protected RNA oligonucleotides, the CE function can be cleaved with tetrabutylammonium fluoride (TBAF) in THF. Indeed, by using 3'-O-(2-cyanoethyl)-2'-deoxythymidine- 5'-phosphate as a simple model compound for cleavage tests, we found out that the 3'-O-CE function is quantitatively cleaved with 1 m TBAF in THF. However, the CE group is also cleaved by other small bases like hydroxy groups under alkaline conditions. The CE cleavage with TBAF in THF gives the fastest and quantitative removal of the CE group under mild conditions for our sequencing-by-synthesis (SBS) application. The efficient removal of the 3'-CE group is crucial for the proof-of-principle of our SBS approach using dye-labeled 3'-CE-blocked dNTPs, which is currently under investigation. Herein we describe the application of 3'-O-(2-cyanoethyl)-2'-deoxythymidine-5'- phosphate as model compound for the development of reversible terminators for the SBS technique. Furthermore we suggest that nucleoside phosphates bearing any removable 3'-modification might be suitable model compounds for cleavage studies in a heterogeneous environment comparable to an oligonucleotide/aprotic solvent system.