862980-74-3Relevant academic research and scientific papers
Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR
May, Jonathan P.,Brown, Lynda J.,Van Delft, Ian,Thelwell, Nicola,Harley, Kate,Brown, Tom
, p. 2534 - 2542 (2005)
A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5′-end, the 3′-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrat
