86324-44-9Relevant academic research and scientific papers
Structural basis for the broad-spectrum inhibition of metallo-β- lactamases by thiols
Lienard, Benoit M. R.,Garau, Gianpiero,Horsfall, Louise,Karsisiotis, Andreas I.,Damblon, Christian,Lassaux, Patricia,Papamicael, Cyril,Roberts, Gordon C. K.,Galleni, Moreno,Dideberg, Otto,Frere, Jean-Marie,Schofield, Christopher J.
scheme or table, p. 2282 - 2294 (2009/02/02)
The development of broad-spectrum metallo-β-lactamase (MBL) inhibitors is challenging due to structural diversity and differences in metal utilisation by these enzymes. Analysis of structural data, followed by non-denturing mass spectrometric analyses, identified thiols proposed to inhibit representative MBLs from all three sub-classes: B1, B2 and B3. Solution analyses led to the identification of broad spectrum inhibitors, including potent inhibitors of the CphA MBL (Aeromonas hydrophila). Structural studies revealed that, as observed for other B1 and B3 MBLs, inhibition of the L1 MBL thiols involves metal chelation. Evidence is reported that this is not the case for inhibition of the CphA enzyme by some thiols; the crystal structure of the CphA-Zn-inhibitor complex reveals a binding mode in which the thiol does not interact with the zinc. The structural data enabled the design and the production of further more potent inhibitors. Overall the results suggest that the development of reasonably broad-spectrum MBL inhibitors should be possible.
Angiotensin converting enzyme inhibitors: N-substituted monocyclic and bicyclic amino acid derivatives
Stanton,Gruenfeld,Babiarz,Ackerman,Friedmann,Yuan,Macchia
, p. 1267 - 1277 (2007/10/02)
The synthesis of N-(3-mercaptopropionyl)-N-arylglycines (14a-x), -N-arylalanines (15a,b), -N-cycloalkylglycines (16a-k), and -1,2,3,4-tetrahydroisoquinoline-3-carboxylic acids (17a-d), -1,2,3,4-tetrahydroquinoline-2-carboxylic acids (18a-f), and -indoline-2-carboxylic acids (19a-k) is described. In vitro inhibition of angiotensin converting enzyme (ACE) is reported for each compound, and the structure-activity relationship for each series is discussed. The in vivo inhibition of ACE and antihypertensive effects of representative compounds from each series are discussed. The most potent compound, 19d, had an in vitro ACE IC50 of 2.6 x 10-9 M and lowered blood pressure in spontaneous hypertensive rats 85 mm at a dose of 10 mg/kg po.
