884347-75-5Relevant academic research and scientific papers
Dda helicase unwinds a DNA-PNA chimeric substrate: Evidence for an inchworm mechanism
Spurling, Travis L.,Eoff, Robert L.,Raney, Kevin D.
, p. 1816 - 1820 (2008/03/13)
Helicases are ubiquitous enzymes involved in all aspects of DNA metabolism including replication, repair, recombination, and transcription. The mechanism of the bacteriophage T4 Dda helicase was investigated by preparing a DNA-PNA chimeric substrate. Surp
Peptide nucleic acids and their phosphonate analogues: Synthesis and hybridization properties
Efimov,Buryakova,Choob,Chakhmakhcheva
, p. 618 - 630 (2007/10/03)
The synthesis of a series of DNA mimics-peptide nucleic acids, phosphonate analogues of peptide nucleic acids, and their hybrids - is described. The preparative synthesis of the corresponding monomers and the solid phase automated synthesis of oligomers-mimics are developed. Modified phosphonate analogues of peptide nucleic acids, in particular, chiral derivatives and those with additional hydroxyl groups in the side chains of the backbone, as well as pyrene derivatives of peptide nucleic acids and their phosphonate analogues, are prepared. The affinity of the resulting oligomers specifically to hybridize to DNA and RNA complementary chains is studied. It is shown that phosphonate analogues of peptide nucleic acids and their hybrids with peptide nucleic acids can form complexes with the DNA and RNA complementary strands, the stability of the complexes increasing in parallel with the increase in the number of peptide nucleic acid residues in the chain of the mimic. This property, along with good water solubility, provides the precondition for further evaluation of these compounds as antisense and antigene agents.
