9001-53-0

Basic information

• Product Name: DIAMINE OXIDASE
• Synonyms: AMINE: OXYGEN OXIDOREDUCTASE[DEAMINATING] [PYRIDOXAL-CONTAINING];EC 1.4.3.6;EC NUMBER: 1.4.3.6;ENZYME COMMISSION NUMBER: 1.4.3.6;DIAMINE OXIDASE;amine oxidase, copper-containing;diamine oxidase from porcine kidney;Oxidase, diamine
• CAS NO: 9001-53-0
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-613-6
• Mol File: 9001-53-0.mol

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• Solubility: 100 mM sodium phosphate buffer, pH 7.2: soluble10mg/mL
• CAS DataBase Reference: DIAMINE OXIDASE(CAS DataBase Reference)
• NIST Chemistry Reference: DIAMINE OXIDASE(9001-53-0)
• EPA Substance Registry System: DIAMINE OXIDASE(9001-53-0)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 9001-53-0(Hazardous Substances Data)

Usage

Uses

Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.

Definition

An enzyme occurring in the animal digestive system; it converts histidine to histamine.

Biological Functions

Diamine oxidase attacks both diamines and histamine in much the same way that MAO attacks monoamines, forming aldehydes. This enzyme is inhibited by carbonyl-blocking reagents and produces hydrogen peroxide, supporting the role of pyridoxal phosphate and the flavin prosthetic groups in the catalytic action of the enzyme. Diamine oxidase is recovered in the supernatant after centrifugation and removal of particulate matter. It is present in kidneys, intestines, liver, lung, and nervous tissue. It limits the biological effects of histamine and the polymethylene amines putrescine and cadaverine. It also attacks monoamines, but at a higher substrate concentration.Plasma amine oxidases are in blood plasma of mammals and include spermine oxidase, which deaminates spermine and other polyamines.

General Description

Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.

Biochem/physiol Actions

Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.